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Title: Multiple Syntrophic Interactions in a Terephthalate-Degrading Methanogenic Consortium

Abstract

Terephthalate (TA) is one of the top 50 chemicals produced worldwide. Its production results in a TA-containing wastewater that is treated by anaerobic processes through a poorly understood methanogenic syntrophy. Using metagenomics, we characterized the methanogenic consortium tinside a hyper-mesophilic (i.e., between mesophilic and thermophilic), TA-degrading bioreactor. We identified genes belonging to dominant Pelotomaculum species presumably involved in TA degradation through decarboxylation, dearomatization, and modified ?-oxidation to H{sub 2}/CO{sub 2} and acetate. These intermediates are converted to CH{sub 4}/CO{sub 2} by three novel hyper-mesophilic methanogens. Additional secondary syntrophic interactions were predicted in Thermotogae, Syntrophus and candidate phyla OP5 and WWE1 populations. The OP5 encodes genes capable of anaerobic autotrophic butyrate production and Thermotogae, Syntrophus and WWE1 have the genetic potential to oxidize butyrate to COsub 2}/H{sub 2} and acetate. These observations suggest that the TA-degrading consortium consists of additional syntrophic interactions beyond the standard H{sub 2}-producing syntroph ? methanogen partnership that may serve to improve community stability.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Genomics Division
OSTI Identifier:
1051790
Report Number(s):
LBNL-5178E
Journal ID: ISSN 1751-7362
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
The ISME Journal
Additional Journal Information:
Journal Volume: 5; Journal Issue: 1; Related Information: Journal Publication Date: 08/05/10; Journal ID: ISSN 1751-7362
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Syntrophic Interactions Terephthalate-Degrading Methanogenic Consortium

Citation Formats

Lykidis, Athanasios, Chen, Chia-Lung, Tringe, Susannah G, McHardy, Alice C, Copeland, Alex 5, Kyrpides, Nikos C, Hugenholtz, Philip, and Liu, Wen-Tso. Multiple Syntrophic Interactions in a Terephthalate-Degrading Methanogenic Consortium. United States: N. p., 2010. Web. doi:10.1038/ismej.2010.125.
Lykidis, Athanasios, Chen, Chia-Lung, Tringe, Susannah G, McHardy, Alice C, Copeland, Alex 5, Kyrpides, Nikos C, Hugenholtz, Philip, & Liu, Wen-Tso. Multiple Syntrophic Interactions in a Terephthalate-Degrading Methanogenic Consortium. United States. https://doi.org/10.1038/ismej.2010.125
Lykidis, Athanasios, Chen, Chia-Lung, Tringe, Susannah G, McHardy, Alice C, Copeland, Alex 5, Kyrpides, Nikos C, Hugenholtz, Philip, and Liu, Wen-Tso. 2010. "Multiple Syntrophic Interactions in a Terephthalate-Degrading Methanogenic Consortium". United States. https://doi.org/10.1038/ismej.2010.125. https://www.osti.gov/servlets/purl/1051790.
@article{osti_1051790,
title = {Multiple Syntrophic Interactions in a Terephthalate-Degrading Methanogenic Consortium},
author = {Lykidis, Athanasios and Chen, Chia-Lung and Tringe, Susannah G and McHardy, Alice C and Copeland, Alex 5 and Kyrpides, Nikos C and Hugenholtz, Philip and Liu, Wen-Tso},
abstractNote = {Terephthalate (TA) is one of the top 50 chemicals produced worldwide. Its production results in a TA-containing wastewater that is treated by anaerobic processes through a poorly understood methanogenic syntrophy. Using metagenomics, we characterized the methanogenic consortium tinside a hyper-mesophilic (i.e., between mesophilic and thermophilic), TA-degrading bioreactor. We identified genes belonging to dominant Pelotomaculum species presumably involved in TA degradation through decarboxylation, dearomatization, and modified ?-oxidation to H{sub 2}/CO{sub 2} and acetate. These intermediates are converted to CH{sub 4}/CO{sub 2} by three novel hyper-mesophilic methanogens. Additional secondary syntrophic interactions were predicted in Thermotogae, Syntrophus and candidate phyla OP5 and WWE1 populations. The OP5 encodes genes capable of anaerobic autotrophic butyrate production and Thermotogae, Syntrophus and WWE1 have the genetic potential to oxidize butyrate to COsub 2}/H{sub 2} and acetate. These observations suggest that the TA-degrading consortium consists of additional syntrophic interactions beyond the standard H{sub 2}-producing syntroph ? methanogen partnership that may serve to improve community stability.},
doi = {10.1038/ismej.2010.125},
url = {https://www.osti.gov/biblio/1051790}, journal = {The ISME Journal},
issn = {1751-7362},
number = 1,
volume = 5,
place = {United States},
year = {Thu Aug 05 00:00:00 EDT 2010},
month = {Thu Aug 05 00:00:00 EDT 2010}
}