Evaluation of Flow-Injection Tandem Mass Spectrometry for Rapid and High-Throughput Quantitative Determination of B-Vitamins in Nutritional Supplements

Journal Article · Sun Jan 01 00:00:00 EST 2012 · Journal of Agricultural and Food Chemistry

DOI:https://doi.org/10.1021/jf302653d· OSTI ID:1049825

Bhandari, Deepak ^[1]; Van Berkel, Gary J ^[1]

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1. ORNL

The use of flow-injection electrospray ionization tandem mass spectrometry for rapid and high-throughput mass spectral analysis of selected B-vitamins, viz. B1, B2, B3, B5, and B6, in nutritional formulations was demonstrated. A simple and rapid (~5 min) in-tube sample preparation was performed by adding extraction solvent to a powdered sample aliquot followed by agitation, centrifugation, and filtration to recover an extract for analysis. Automated flow injection introduced 1 L of the extracts directly into the mass spectrometer ion source without chromatographic separation. Sample-to-sample analysis time was 60 s representing significant improvement over conventional liquid chromatography approaches which typically require 25-45 min, and often require more significant sample preparation procedures. Quantitative capabilities of the flow-injection analysis were tested using the method of standard additions and NIST standard reference material (SRM 3280) multivitamin/multielement tablets. The quantity determined for each B-vitamin in SRM 3280 was within the statistical range provided for the respective certified values. The same sample preparation and analysis approach was also applied to two different commercial vitamin supplement tablets and proved to be successful in the quantification of the selected B-vitamins as evidenced by an agreement with the labels values and the results obtained using isotope dilution liquid chromatography/mass spectrometry.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1049825
• Journal Information: Journal of Agricultural and Food Chemistry, Vol. 60, Issue 34; ISSN 0021-8561
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
CALIBRATION STANDARDS
CENTRIFUGATION
CHROMATOGRAPHY
EVALUATION
FILTRATION
ION SOURCES
IONIZATION
ISOTOPE DILUTION
MASS SPECTROMETERS
MASS SPECTROSCOPY
SAMPLE PREPARATION
SOLVENTS
SPECTROSCOPY
VITAMINS
Solid-liquid extraction
flow-injection
tandem mass spectrometry
electrospray ionization
B-vitamins
quantitative analysis