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Title: Automatic Detection, Segmentation and Classification of Retinal Horizontal Neurons in Large-scale 3D Confocal Imagery

Abstract

Automatic analysis of neuronal structure from wide-field-of-view 3D image stacks of retinal neurons is essential for statistically characterizing neuronal abnormalities that may be causally related to neural malfunctions or may be early indicators for a variety of neuropathies. In this paper, we study classification of neuron fields in large-scale 3D confocal image stacks, a challenging neurobiological problem because of the low spatial resolution imagery and presence of intertwined dendrites from different neurons. We present a fully automated, four-step processing approach for neuron classification with respect to the morphological structure of their dendrites. In our approach, we first localize each individual soma in the image by using morphological operators and active contours. By using each soma position as a seed point, we automatically determine an appropriate threshold to segment dendrites of each neuron. We then use skeletonization and network analysis to generate the morphological structures of segmented dendrites, and shape-based features are extracted from network representations of each neuron to characterize the neuron. Based on qualitative results and quantitative comparisons, we show that we are able to automatically compute relevant features that clearly distinguish between normal and abnormal cases for postnatal day 6 (P6) horizontal neurons.

Authors:
 [1];  [1];  [1];  [2];  [2]
  1. ORNL
  2. St. Jude Children's Research Hospital
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
Work for Others (WFO)
OSTI Identifier:
1049193
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Conference
Resource Relation:
Conference: SPIE Medical Imaging 2011, Orlando, FL, USA, 20110307, 20110307
Country of Publication:
United States
Language:
English
Subject:
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CLASSIFICATION; DENDRITES; DETECTION; NERVE CELLS; NETWORK ANALYSIS; PROCESSING; SEEDS; SPATIAL RESOLUTION

Citation Formats

Karakaya, Mahmut, Kerekes, Ryan A, Gleason, Shaun Scott, Martins, Rodrigo, and Dyer, Michael. Automatic Detection, Segmentation and Classification of Retinal Horizontal Neurons in Large-scale 3D Confocal Imagery. United States: N. p., 2011. Web.
Karakaya, Mahmut, Kerekes, Ryan A, Gleason, Shaun Scott, Martins, Rodrigo, & Dyer, Michael. Automatic Detection, Segmentation and Classification of Retinal Horizontal Neurons in Large-scale 3D Confocal Imagery. United States.
Karakaya, Mahmut, Kerekes, Ryan A, Gleason, Shaun Scott, Martins, Rodrigo, and Dyer, Michael. 2011. "Automatic Detection, Segmentation and Classification of Retinal Horizontal Neurons in Large-scale 3D Confocal Imagery". United States.
@article{osti_1049193,
title = {Automatic Detection, Segmentation and Classification of Retinal Horizontal Neurons in Large-scale 3D Confocal Imagery},
author = {Karakaya, Mahmut and Kerekes, Ryan A and Gleason, Shaun Scott and Martins, Rodrigo and Dyer, Michael},
abstractNote = {Automatic analysis of neuronal structure from wide-field-of-view 3D image stacks of retinal neurons is essential for statistically characterizing neuronal abnormalities that may be causally related to neural malfunctions or may be early indicators for a variety of neuropathies. In this paper, we study classification of neuron fields in large-scale 3D confocal image stacks, a challenging neurobiological problem because of the low spatial resolution imagery and presence of intertwined dendrites from different neurons. We present a fully automated, four-step processing approach for neuron classification with respect to the morphological structure of their dendrites. In our approach, we first localize each individual soma in the image by using morphological operators and active contours. By using each soma position as a seed point, we automatically determine an appropriate threshold to segment dendrites of each neuron. We then use skeletonization and network analysis to generate the morphological structures of segmented dendrites, and shape-based features are extracted from network representations of each neuron to characterize the neuron. Based on qualitative results and quantitative comparisons, we show that we are able to automatically compute relevant features that clearly distinguish between normal and abnormal cases for postnatal day 6 (P6) horizontal neurons.},
doi = {},
url = {https://www.osti.gov/biblio/1049193}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2011},
month = {Sat Jan 01 00:00:00 EST 2011}
}

Conference:
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