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Title: Visualizing Macromolecular Complexes with In Situ Liquid Scanning Transmission Electron Microscopy

Abstract

A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1048604
Report Number(s):
PNNL-SA-85511
Journal ID: ISSN 0047-7206; MICOB5; TRN: US201216%%1037
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Micron, 43(11):1085–1090
Additional Journal Information:
Journal Volume: 43; Journal Issue: 11; Journal ID: ISSN 0047-7206
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BIOLOGY; PROTEINS; TRANSMISSION ELECTRON MICROSCOPY; In situ TEM; Liquid TEM; Nanolipoprotein; Aberration correct

Citation Formats

Evans, James E., Jungjohann, K. L., Wong, Peony C. K., Chiu, Po-Lin, Dutrow, Gavin H., Arslan, Ilke, and Browning, Nigel D. Visualizing Macromolecular Complexes with In Situ Liquid Scanning Transmission Electron Microscopy. United States: N. p., 2012. Web. doi:10.1016/j.micron.2012.01.018.
Evans, James E., Jungjohann, K. L., Wong, Peony C. K., Chiu, Po-Lin, Dutrow, Gavin H., Arslan, Ilke, & Browning, Nigel D. Visualizing Macromolecular Complexes with In Situ Liquid Scanning Transmission Electron Microscopy. United States. doi:10.1016/j.micron.2012.01.018.
Evans, James E., Jungjohann, K. L., Wong, Peony C. K., Chiu, Po-Lin, Dutrow, Gavin H., Arslan, Ilke, and Browning, Nigel D. Thu . "Visualizing Macromolecular Complexes with In Situ Liquid Scanning Transmission Electron Microscopy". United States. doi:10.1016/j.micron.2012.01.018.
@article{osti_1048604,
title = {Visualizing Macromolecular Complexes with In Situ Liquid Scanning Transmission Electron Microscopy},
author = {Evans, James E. and Jungjohann, K. L. and Wong, Peony C. K. and Chiu, Po-Lin and Dutrow, Gavin H. and Arslan, Ilke and Browning, Nigel D.},
abstractNote = {A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.},
doi = {10.1016/j.micron.2012.01.018},
journal = {Micron, 43(11):1085–1090},
issn = {0047-7206},
number = 11,
volume = 43,
place = {United States},
year = {2012},
month = {11}
}