skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Harnessing glycosylation to improve cellulase activity

Abstract

Cellulases and hemicellulases are responsible for the turnover of plant cell wall polysaccharides in the biosphere, and thus form the foundation of enzyme engineering efforts in biofuels research. Many of these carbohydrate-active enzymes from filamentous fungi contain both N-linked and O-linked glycosylation, the extent and heterogeneity of which depends on growth conditions, expression host, and the presence of glycan trimming enzymes in the secretome, all of which in turn impacts enzyme activity. As the roles of glycosylation in enzyme function have not been fully elucidated, here we discuss the potential roles of glycosylation on glycoside hydrolase enzyme structure and function after secretion. We posit that glycosylation, instead of hindering cellulase engineering, can be used as an additional tool to enhance enzyme activity, given deeper understanding of its molecular-level role in biomass deconstruction.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1048603
Report Number(s):
PNNL-SA-83208
BM0101020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Current Opinion in Biotechnology, 23(3):338-345
Additional Journal Information:
Journal Name: Current Opinion in Biotechnology, 23(3):338-345
Country of Publication:
United States
Language:
English
Subject:
Cellulase, Glycosylation, Filamentous fungi

Citation Formats

Beckham, Gregg T., Dai, Ziyu, Mattews, James F., Momany, Michelle, Payne, Christina M., Adney, William S., Baker, Scott E., and Himmel, Michael E. Harnessing glycosylation to improve cellulase activity. United States: N. p., 2012. Web. doi:10.1016/j.copbio.2011.11.030.
Beckham, Gregg T., Dai, Ziyu, Mattews, James F., Momany, Michelle, Payne, Christina M., Adney, William S., Baker, Scott E., & Himmel, Michael E. Harnessing glycosylation to improve cellulase activity. United States. doi:10.1016/j.copbio.2011.11.030.
Beckham, Gregg T., Dai, Ziyu, Mattews, James F., Momany, Michelle, Payne, Christina M., Adney, William S., Baker, Scott E., and Himmel, Michael E. Mon . "Harnessing glycosylation to improve cellulase activity". United States. doi:10.1016/j.copbio.2011.11.030.
@article{osti_1048603,
title = {Harnessing glycosylation to improve cellulase activity},
author = {Beckham, Gregg T. and Dai, Ziyu and Mattews, James F. and Momany, Michelle and Payne, Christina M. and Adney, William S. and Baker, Scott E. and Himmel, Michael E.},
abstractNote = {Cellulases and hemicellulases are responsible for the turnover of plant cell wall polysaccharides in the biosphere, and thus form the foundation of enzyme engineering efforts in biofuels research. Many of these carbohydrate-active enzymes from filamentous fungi contain both N-linked and O-linked glycosylation, the extent and heterogeneity of which depends on growth conditions, expression host, and the presence of glycan trimming enzymes in the secretome, all of which in turn impacts enzyme activity. As the roles of glycosylation in enzyme function have not been fully elucidated, here we discuss the potential roles of glycosylation on glycoside hydrolase enzyme structure and function after secretion. We posit that glycosylation, instead of hindering cellulase engineering, can be used as an additional tool to enhance enzyme activity, given deeper understanding of its molecular-level role in biomass deconstruction.},
doi = {10.1016/j.copbio.2011.11.030},
journal = {Current Opinion in Biotechnology, 23(3):338-345},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {6}
}