skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A model for improving microbial biofuel production using a synthetic feedback loop

Abstract

Cells use feedback to implement a diverse range of regulatory functions. Building synthetic feedback control systems may yield insight into the roles that feedback can play in regulation since it can be introduced independently of native regulation, and alternative control architectures can be compared. We propose a model for microbial biofuel production where a synthetic control system is used to increase cell viability and biofuel yields. Although microbes can be engineered to produce biofuels, the fuels are often toxic to cell growth, creating a negative feedback loop that limits biofuel production. These toxic effects may be mitigated by expressing efflux pumps that export biofuel from the cell. We developed a model for cell growth and biofuel production and used it to compare several genetic control strategies for their ability to improve biofuel yields. We show that controlling efflux pump expression directly with a biofuel-responsive promoter is a straight forward way of improving biofuel production. In addition, a feed forward loop controller is shown to be versatile at dealing with uncertainty in biofuel production rates.

Authors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Physical Biosciences Division
OSTI Identifier:
1048307
Report Number(s):
LBNL-4988E
TRN: US201216%%884
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
Synthetic and Systems Biology
Additional Journal Information:
Journal Volume: 4; Related Information: Journal Publication Date: 25 FEB 2010
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; BIOFUELS; CONTROL SYSTEMS; EXPORTS; FEEDBACK; GENETIC CONTROL; PRODUCTION; PROMOTERS; REGULATIONS; VIABILITY

Citation Formats

Dunlop, Mary, Keasling, Jay, and Mukhopadhyay, Aindrila. A model for improving microbial biofuel production using a synthetic feedback loop. United States: N. p., 2011. Web.
Dunlop, Mary, Keasling, Jay, & Mukhopadhyay, Aindrila. A model for improving microbial biofuel production using a synthetic feedback loop. United States.
Dunlop, Mary, Keasling, Jay, and Mukhopadhyay, Aindrila. Thu . "A model for improving microbial biofuel production using a synthetic feedback loop". United States. https://www.osti.gov/servlets/purl/1048307.
@article{osti_1048307,
title = {A model for improving microbial biofuel production using a synthetic feedback loop},
author = {Dunlop, Mary and Keasling, Jay and Mukhopadhyay, Aindrila},
abstractNote = {Cells use feedback to implement a diverse range of regulatory functions. Building synthetic feedback control systems may yield insight into the roles that feedback can play in regulation since it can be introduced independently of native regulation, and alternative control architectures can be compared. We propose a model for microbial biofuel production where a synthetic control system is used to increase cell viability and biofuel yields. Although microbes can be engineered to produce biofuels, the fuels are often toxic to cell growth, creating a negative feedback loop that limits biofuel production. These toxic effects may be mitigated by expressing efflux pumps that export biofuel from the cell. We developed a model for cell growth and biofuel production and used it to compare several genetic control strategies for their ability to improve biofuel yields. We show that controlling efflux pump expression directly with a biofuel-responsive promoter is a straight forward way of improving biofuel production. In addition, a feed forward loop controller is shown to be versatile at dealing with uncertainty in biofuel production rates.},
doi = {},
journal = {Synthetic and Systems Biology},
number = ,
volume = 4,
place = {United States},
year = {2011},
month = {7}
}