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Title: Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata

Abstract

The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of {approx}456 polypeptide chains contributed by {approx}30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N-terminal 'FG' repeats containing a Gle2p-binding sequence motif and a NPC targeting domain at its C-terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882-1034 [CgNup116(882-1034)], at 1.94 {angstrom} resolution. The X-ray structure of CgNup116(882-1034) is consistent with the molecular envelope determined in solution by small-angle X-ray scattering. Structural similarities of CgNup116(882-1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed.

Authors:
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  1. (Einstein)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
DOE - BASIC ENERGY SCIENCESNIH
OSTI Identifier:
1047886
Resource Type:
Journal Article
Journal Name:
Proteins
Additional Journal Information:
Journal Volume: 80; Journal Issue: (8) ; 08, 2012; Journal ID: ISSN 0887-3585
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; CANDIDA; CRYSTAL STRUCTURE; CYTOPLASM; EXPORTS; FUNGI; IMPORTS; POLYPEPTIDES; PROTEINS; RESIDUES; RESOLUTION; SACCHAROMYCES CEREVISIAE; SCATTERING; YEASTS

Citation Formats

Sampathkumar, Parthasarathy, Kim, Seung Joong, Manglicmot, Danalyn, Bain, Kevin T., Gilmore, Jeremiah, Gheyi, Tarun, Phillips, Jeremy, Pieper, Ursula, Fernandez-Martinez, Javier, Franke, Josef D., Matsui, Tsutomu, Tsuruta, Hiro, Atwell, Shane, Thompson, Devon A., Emtage, J. Spencer, Wasserman, Stephen R., Rout, Michael P., Sali, Andrej, Sauder, J. Michael, Almo, Steven C., Burley, Stephen K., SLAC), Rockefeller), UCSF), and Lilly). Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata. United States: N. p., 2012. Web. doi:10.1002/prot.24102.
Sampathkumar, Parthasarathy, Kim, Seung Joong, Manglicmot, Danalyn, Bain, Kevin T., Gilmore, Jeremiah, Gheyi, Tarun, Phillips, Jeremy, Pieper, Ursula, Fernandez-Martinez, Javier, Franke, Josef D., Matsui, Tsutomu, Tsuruta, Hiro, Atwell, Shane, Thompson, Devon A., Emtage, J. Spencer, Wasserman, Stephen R., Rout, Michael P., Sali, Andrej, Sauder, J. Michael, Almo, Steven C., Burley, Stephen K., SLAC), Rockefeller), UCSF), & Lilly). Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata. United States. doi:10.1002/prot.24102.
Sampathkumar, Parthasarathy, Kim, Seung Joong, Manglicmot, Danalyn, Bain, Kevin T., Gilmore, Jeremiah, Gheyi, Tarun, Phillips, Jeremy, Pieper, Ursula, Fernandez-Martinez, Javier, Franke, Josef D., Matsui, Tsutomu, Tsuruta, Hiro, Atwell, Shane, Thompson, Devon A., Emtage, J. Spencer, Wasserman, Stephen R., Rout, Michael P., Sali, Andrej, Sauder, J. Michael, Almo, Steven C., Burley, Stephen K., SLAC), Rockefeller), UCSF), and Lilly). Tue . "Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata". United States. doi:10.1002/prot.24102.
@article{osti_1047886,
title = {Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata},
author = {Sampathkumar, Parthasarathy and Kim, Seung Joong and Manglicmot, Danalyn and Bain, Kevin T. and Gilmore, Jeremiah and Gheyi, Tarun and Phillips, Jeremy and Pieper, Ursula and Fernandez-Martinez, Javier and Franke, Josef D. and Matsui, Tsutomu and Tsuruta, Hiro and Atwell, Shane and Thompson, Devon A. and Emtage, J. Spencer and Wasserman, Stephen R. and Rout, Michael P. and Sali, Andrej and Sauder, J. Michael and Almo, Steven C. and Burley, Stephen K. and SLAC) and Rockefeller) and UCSF) and Lilly)},
abstractNote = {The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of {approx}456 polypeptide chains contributed by {approx}30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N-terminal 'FG' repeats containing a Gle2p-binding sequence motif and a NPC targeting domain at its C-terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882-1034 [CgNup116(882-1034)], at 1.94 {angstrom} resolution. The X-ray structure of CgNup116(882-1034) is consistent with the molecular envelope determined in solution by small-angle X-ray scattering. Structural similarities of CgNup116(882-1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed.},
doi = {10.1002/prot.24102},
journal = {Proteins},
issn = {0887-3585},
number = (8) ; 08, 2012,
volume = 80,
place = {United States},
year = {2012},
month = {10}
}