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Title: THE ROLE FUNGI AND YEAST IN MONITORED NATURAL ATTENUATION

Abstract

Fungi and yeast have been characterized as important components in the bioremediation of organic contaminants in soil and water including polyaromatic hydrocarbons (PAHs); however, research into their ability to metabolize these compounds in extreme environments has been limited. In this work forty-three fungi and yeasts were isolated from a PAH-contaminated sludge waste lagoon in Poland. The lagoon was part of a monitored natural attenuation (MNA) study where natural reduction of PAHs and associated toxicity over time in non-disturbed areas of the sludge lagoon indicated MNA activity. The microorganisms were initially isolated on minimal medium containing naphthalene as the sole carbon and energy source. Fungal isolates were then maintained on MEA and identified based on microscopic examination and BIOLOG{reg_sign}. The analysis identified several of the fungal isolates as belonging to the genera Penicillium, Paecilomyces, Aspergillus, and Eupenicillium. Yeasts included Candida parapsilosis and C. fluvialitis. Further microbial characterization revealed that several isolates were capable of rowing on acidified media of pH 4, 3, and 2.5. Over twenty percent of the fungi demonstrated growth as low as pH 2.5. Of the 43 isolates examined, 24 isolates exhibited growth at 5 C. Nine of the fungal isolates exhibiting growth at 5 C were thenmore » examined for metabolic activity using a respirometer testing metabolic activity at pH 3. Microcosm studies confirmed the growth of the fungi on PAH contaminated sediment as the sole carbon and energy source with elevated metabolic rates indicating evidence of MNA. Our findings suggest that many of the Poland fungal isolates may be of value in the bioremediation processes in acidic waste sites in northern climates typical of Northern Europe.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
SRS
Sponsoring Org.:
USDOE
OSTI Identifier:
1046156
Report Number(s):
SRNL-STI-2010-00036
TRN: US201216%%91
DOE Contract Number:  
DE-AC09-08SR22470
Resource Type:
Book
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIOREMEDIATION; FUNGI; GROWTH; NATURAL ATTENUATION; POLYCYCLIC AROMATIC HYDROCARBONS; SLUDGES; SOILS; WATER; YEASTS

Citation Formats

Brigmon, R., Abe, M., Johnson, B., Simpson, W., and Mckinsey, P. THE ROLE FUNGI AND YEAST IN MONITORED NATURAL ATTENUATION. United States: N. p., 2010. Web.
Brigmon, R., Abe, M., Johnson, B., Simpson, W., & Mckinsey, P. THE ROLE FUNGI AND YEAST IN MONITORED NATURAL ATTENUATION. United States.
Brigmon, R., Abe, M., Johnson, B., Simpson, W., and Mckinsey, P. Tue . "THE ROLE FUNGI AND YEAST IN MONITORED NATURAL ATTENUATION". United States. https://www.osti.gov/servlets/purl/1046156.
@article{osti_1046156,
title = {THE ROLE FUNGI AND YEAST IN MONITORED NATURAL ATTENUATION},
author = {Brigmon, R. and Abe, M. and Johnson, B. and Simpson, W. and Mckinsey, P.},
abstractNote = {Fungi and yeast have been characterized as important components in the bioremediation of organic contaminants in soil and water including polyaromatic hydrocarbons (PAHs); however, research into their ability to metabolize these compounds in extreme environments has been limited. In this work forty-three fungi and yeasts were isolated from a PAH-contaminated sludge waste lagoon in Poland. The lagoon was part of a monitored natural attenuation (MNA) study where natural reduction of PAHs and associated toxicity over time in non-disturbed areas of the sludge lagoon indicated MNA activity. The microorganisms were initially isolated on minimal medium containing naphthalene as the sole carbon and energy source. Fungal isolates were then maintained on MEA and identified based on microscopic examination and BIOLOG{reg_sign}. The analysis identified several of the fungal isolates as belonging to the genera Penicillium, Paecilomyces, Aspergillus, and Eupenicillium. Yeasts included Candida parapsilosis and C. fluvialitis. Further microbial characterization revealed that several isolates were capable of rowing on acidified media of pH 4, 3, and 2.5. Over twenty percent of the fungi demonstrated growth as low as pH 2.5. Of the 43 isolates examined, 24 isolates exhibited growth at 5 C. Nine of the fungal isolates exhibiting growth at 5 C were then examined for metabolic activity using a respirometer testing metabolic activity at pH 3. Microcosm studies confirmed the growth of the fungi on PAH contaminated sediment as the sole carbon and energy source with elevated metabolic rates indicating evidence of MNA. Our findings suggest that many of the Poland fungal isolates may be of value in the bioremediation processes in acidic waste sites in northern climates typical of Northern Europe.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {1}
}

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