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Title: The 2.3-Angstrom Structure of Porcine Circovirus 2

Abstract

Porcine circovirus 2 (PCV2) is a T = 1 nonenveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3-{angstrom} resolution, and the cryo-electron microscopy (cryo-EM) image reconstruction of a full-length PCV2 virus-like particle at 9.6-{angstrom} resolution. This is the first atomic structure of a circovirus. The crystal structure revealed that the capsid protein fold is a canonical viral jelly roll. The loops connecting the strands of the jelly roll define the limited features of the surface. Sulfate ions interacting with the surface and electrostatic potential calculations strongly suggest a heparan sulfate binding site that allows PCV2 to gain entry into the cell. The crystal structure also allowed previously determined epitopes of the capsid to be visualized. The cryo-EM image reconstruction showed that the location of the N terminus, absent in the crystal structure, is inside the capsid. As the N terminus was previously shown to be antigenic, it may externalize through viral 'breathing'.

Authors:
; ; ; ; ; ;  [1];  [2]
  1. (Scripps)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
INDUSTRYNIH
OSTI Identifier:
1045683
Resource Type:
Journal Article
Journal Name:
J. Virol.
Additional Journal Information:
Journal Volume: 85; Journal Issue: (15) ; 08, 2011; Journal ID: ISSN 0022-538X
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; CRYSTAL STRUCTURE; ELECTROSTATICS; MICROSCOPY; PROTEINS; RESOLUTION; RESPIRATION; SULFATES; SWINE

Citation Formats

Khayat, Reza, Brunn, Nicholas, Speir, Jeffrey A., Hardham, John M., Ankenbauer, Robert G., Schneemann, Anette, Johnson, John E., and Pfizer). The 2.3-Angstrom Structure of Porcine Circovirus 2. United States: N. p., 2012. Web. doi:10.1128/JVI.00737-11.
Khayat, Reza, Brunn, Nicholas, Speir, Jeffrey A., Hardham, John M., Ankenbauer, Robert G., Schneemann, Anette, Johnson, John E., & Pfizer). The 2.3-Angstrom Structure of Porcine Circovirus 2. United States. doi:10.1128/JVI.00737-11.
Khayat, Reza, Brunn, Nicholas, Speir, Jeffrey A., Hardham, John M., Ankenbauer, Robert G., Schneemann, Anette, Johnson, John E., and Pfizer). Thu . "The 2.3-Angstrom Structure of Porcine Circovirus 2". United States. doi:10.1128/JVI.00737-11.
@article{osti_1045683,
title = {The 2.3-Angstrom Structure of Porcine Circovirus 2},
author = {Khayat, Reza and Brunn, Nicholas and Speir, Jeffrey A. and Hardham, John M. and Ankenbauer, Robert G. and Schneemann, Anette and Johnson, John E. and Pfizer)},
abstractNote = {Porcine circovirus 2 (PCV2) is a T = 1 nonenveloped icosahedral virus that has had severe impact on the swine industry. Here we report the crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3-{angstrom} resolution, and the cryo-electron microscopy (cryo-EM) image reconstruction of a full-length PCV2 virus-like particle at 9.6-{angstrom} resolution. This is the first atomic structure of a circovirus. The crystal structure revealed that the capsid protein fold is a canonical viral jelly roll. The loops connecting the strands of the jelly roll define the limited features of the surface. Sulfate ions interacting with the surface and electrostatic potential calculations strongly suggest a heparan sulfate binding site that allows PCV2 to gain entry into the cell. The crystal structure also allowed previously determined epitopes of the capsid to be visualized. The cryo-EM image reconstruction showed that the location of the N terminus, absent in the crystal structure, is inside the capsid. As the N terminus was previously shown to be antigenic, it may externalize through viral 'breathing'.},
doi = {10.1128/JVI.00737-11},
journal = {J. Virol.},
issn = {0022-538X},
number = (15) ; 08, 2011,
volume = 85,
place = {United States},
year = {2012},
month = {10}
}