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Title: Monodispersed biocompatible silver sulfide nanoparticles: Facile extracellular biosynthesis using the gamma-proteobacterium, Shewanella oneidensis

Abstract

Interest in engineered metal and semiconductor nanocrystallites continues to grow due to their unique size- and shape-dependent optoelectronic, physicochemical and biological properties. Therefore identifying novel non-hazardous nanoparticle synthesis routes that address hydrophilicity, size and shape control and production costs has become a priority. In the present article we report for the first time on the efficient generation of extracellular silver sulfide (Ag{sub 2}S) nanoparticles by the metal-reducing bacterium Shewanella oneidensis. The particles are reasonably monodispersed and homogeneously shaped. They are produced under ambient temperatures and pressures at high yield, 85% theoretical maximum. UV-visible and Fourier transform infrared spectroscopy, dynamic light scattering, X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy measurements confirmed the formation, optical and surface properties, purity and crystallinity of the synthesized particles. Further characterization revealed that the particles consist of spheres with a mean diameter of 9 {+-} 3.5 nm, and are capped by a detachable protein/peptide surface coat. Toxicity assessments of these biogenic Ag{sub 2}S nanoparticles on Gram-negative (Escherichia coli and S. oneidensis) and Gram-positive (Bacillus subtilis) bacterial systems, as well as eukaryotic cell lines including mouse lung epithelial (C 10) and macrophage (RAW-264.7) cells, showed that the particles were non-inhibitory and non-cytotoxic to any ofmore » these systems. Our results provide a facile, eco-friendly and economical route for the fabrication of technologically important semiconducting Ag{sub 2}S nanoparticles. These particles are dispersible and biocompatible, thus providing excellent potential for use in optical imaging, electronic devices and solar cell applications.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1045295
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Acta Biomaterialia
Additional Journal Information:
Journal Volume: 7; Journal Issue: 12
Country of Publication:
United States
Language:
English
Subject:
14 SOLAR ENERGY; AMBIENT TEMPERATURE; BACILLUS; BIOSYNTHESIS; FABRICATION; LIGHT SCATTERING; LUNGS; MACROPHAGES; PRODUCTION; SHAPE; SILVER SULFIDES; SOLAR CELLS; SPECTROSCOPY; SURFACE PROPERTIES; SYNTHESIS; TOXICITY; TRANSMISSION ELECTRON MICROSCOPY; X-RAY DIFFRACTION; X-RAY PHOTOELECTRON SPECTROSCOPY

Citation Formats

Doktycz, Mitchel John, Moon, Ji Won, Meyer, III, Harry M, Hensley, Dale K, Phelps, Tommy Joe, and Pelletier, Dale A. Monodispersed biocompatible silver sulfide nanoparticles: Facile extracellular biosynthesis using the gamma-proteobacterium, Shewanella oneidensis. United States: N. p., 2011. Web.
Doktycz, Mitchel John, Moon, Ji Won, Meyer, III, Harry M, Hensley, Dale K, Phelps, Tommy Joe, & Pelletier, Dale A. Monodispersed biocompatible silver sulfide nanoparticles: Facile extracellular biosynthesis using the gamma-proteobacterium, Shewanella oneidensis. United States.
Doktycz, Mitchel John, Moon, Ji Won, Meyer, III, Harry M, Hensley, Dale K, Phelps, Tommy Joe, and Pelletier, Dale A. 2011. "Monodispersed biocompatible silver sulfide nanoparticles: Facile extracellular biosynthesis using the gamma-proteobacterium, Shewanella oneidensis". United States.
@article{osti_1045295,
title = {Monodispersed biocompatible silver sulfide nanoparticles: Facile extracellular biosynthesis using the gamma-proteobacterium, Shewanella oneidensis},
author = {Doktycz, Mitchel John and Moon, Ji Won and Meyer, III, Harry M and Hensley, Dale K and Phelps, Tommy Joe and Pelletier, Dale A},
abstractNote = {Interest in engineered metal and semiconductor nanocrystallites continues to grow due to their unique size- and shape-dependent optoelectronic, physicochemical and biological properties. Therefore identifying novel non-hazardous nanoparticle synthesis routes that address hydrophilicity, size and shape control and production costs has become a priority. In the present article we report for the first time on the efficient generation of extracellular silver sulfide (Ag{sub 2}S) nanoparticles by the metal-reducing bacterium Shewanella oneidensis. The particles are reasonably monodispersed and homogeneously shaped. They are produced under ambient temperatures and pressures at high yield, 85% theoretical maximum. UV-visible and Fourier transform infrared spectroscopy, dynamic light scattering, X-ray diffraction, transmission electron microscopy and X-ray photoelectron spectroscopy measurements confirmed the formation, optical and surface properties, purity and crystallinity of the synthesized particles. Further characterization revealed that the particles consist of spheres with a mean diameter of 9 {+-} 3.5 nm, and are capped by a detachable protein/peptide surface coat. Toxicity assessments of these biogenic Ag{sub 2}S nanoparticles on Gram-negative (Escherichia coli and S. oneidensis) and Gram-positive (Bacillus subtilis) bacterial systems, as well as eukaryotic cell lines including mouse lung epithelial (C 10) and macrophage (RAW-264.7) cells, showed that the particles were non-inhibitory and non-cytotoxic to any of these systems. Our results provide a facile, eco-friendly and economical route for the fabrication of technologically important semiconducting Ag{sub 2}S nanoparticles. These particles are dispersible and biocompatible, thus providing excellent potential for use in optical imaging, electronic devices and solar cell applications.},
doi = {},
url = {https://www.osti.gov/biblio/1045295}, journal = {Acta Biomaterialia},
number = 12,
volume = 7,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2011},
month = {Sat Jan 01 00:00:00 EST 2011}
}