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Title: Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures

Abstract

We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 {angstrom} resolution crystal structures.

Authors:
; ; ; ; ; ; ; ;  [1];  [2]
  1. (Labcyte)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
NIHDOE - BIOLOGICAL AND ENVIRONMENTAL RESEARCHNIGMSDOE - OTHER
OSTI Identifier:
1045034
Resource Type:
Journal Article
Journal Name:
Biochemistry-US
Additional Journal Information:
Journal Volume: 50; Journal Issue: (21) ; 05, 2011; Journal ID: ISSN 0006-2960
Country of Publication:
United States
Language:
ENGLISH
Subject:
58 GEOSCIENCES; ACOUSTICS; AIR; CRYSTAL STRUCTURE; RESOLUTION; SLURRIES; X-RAY DIFFRACTION

Citation Formats

Soares, Alexei S., Engel, Matthew A., Stearns, Richard, Datwani, Sammy, Olechno, Joe, Ellson, Richard, Skinner, John M., Allaire, Marc, Orville, Allen M., and BNL). Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures. United States: N. p., 2012. Web. doi:10.1021/bi200549x.
Soares, Alexei S., Engel, Matthew A., Stearns, Richard, Datwani, Sammy, Olechno, Joe, Ellson, Richard, Skinner, John M., Allaire, Marc, Orville, Allen M., & BNL). Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures. United States. doi:10.1021/bi200549x.
Soares, Alexei S., Engel, Matthew A., Stearns, Richard, Datwani, Sammy, Olechno, Joe, Ellson, Richard, Skinner, John M., Allaire, Marc, Orville, Allen M., and BNL). Thu . "Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures". United States. doi:10.1021/bi200549x.
@article{osti_1045034,
title = {Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures},
author = {Soares, Alexei S. and Engel, Matthew A. and Stearns, Richard and Datwani, Sammy and Olechno, Joe and Ellson, Richard and Skinner, John M. and Allaire, Marc and Orville, Allen M. and BNL)},
abstractNote = {We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 {angstrom} resolution crystal structures.},
doi = {10.1021/bi200549x},
journal = {Biochemistry-US},
issn = {0006-2960},
number = (21) ; 05, 2011,
volume = 50,
place = {United States},
year = {2012},
month = {10}
}