Acoustically Mounted Microcrystals Yield High-Resolution X-ray Structures
- Labcyte
We demonstrate a general strategy for determining structures from showers of microcrystals. It uses acoustic droplet ejection to transfer 2.5 nL droplets from the surface of microcrystal slurries, through the air, onto mounting micromesh pins. Individual microcrystals are located by raster-scanning a several-micrometer X-ray beam across the cryocooled micromeshes. X-ray diffraction data sets merged from several micrometer-sized crystals are used to determine 1.8 {angstrom} resolution crystal structures.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- NIHDOE - BIOLOGICAL AND ENVIRONMENTAL RESEARCHNIGMSDOE - OTHER
- OSTI ID:
- 1045034
- Journal Information:
- Biochemistry-US, Vol. 50, Issue (21) ; 05, 2011; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- ENGLISH
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