Overexpression of DMP1 accelerates mineralization and alters cortical bone biomechanical properties in vivo
Dentin matrix protein-1 (DMP1) is a key regulator of biomineralization. Here, we examine changes in structural, geometric, and material properties of cortical bone in a transgenic mouse model overexpressing DMP1. Micro-computed tomography and three-point bending were performed on 90 femora of wild type and transgenic mice at 1, 2, 4, and 6 months. Fourier transform infrared imaging was performed at 2 months. We found that the transgenic femurs were longer (p < 0.01), more robust in cross-section (p < 0.05), stronger (p < 0.05), but had less post-yield strain and displacement (p < 0.01), and higher tissue mineral density (p < 0.01) than the wild type femurs at 1 and 2 months. At 2 months, the transgenic femurs also had a higher mineral-to-matrix ratio (p < 0.05) and lower carbonate substitution (p < 0.05) compared to wild type femurs. These findings indicate that increased mineralization caused by overexpressing DMP1 led to increased structural cortical bone properties associated with decreased ductility during the early post-natal period.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Organization:
- USDOE SC OFFICE OF SCIENCE (SC)
- DOE Contract Number:
- DE-AC02-98CH10886
- OSTI ID:
- 1044008
- Report Number(s):
- BNL-97135-2012-JA; R&D Project: LS001; TRN: US201214%%263
- Journal Information:
- Journal of the Mechanical Behavior of Biomedical Materials, Vol. 5, Issue 1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Nuclear localization of DMP1 proteins suggests a role in intracellular signaling
Osteoporosis Treatments Affect Bone Matrix Maturation in a Rat Model of Induced Cortical Remodeling