skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nanometer to Millimeter Scale Peptide-Porphyrin Materials

Abstract

AQ-Pal14 is a 30-residue polypeptide that was designed to form an {alpha}-helical coiled coil that contains a metal-binding 4-pyridylalanine residue on its solvent-exposed surface. However, characterization of this peptide shows that it exists as a three-stranded coiled coil, not a two-stranded one as predicted from its design. Reaction with cobalt(III) protoporphyrin IX (Co-PPIX) produces a six-coordinate Co-PPIX(AQ-Pal14){sub 2} species that creates two coiled-coil oligomerization domains coordinated to opposite faces of the porphyrin ring. It is found that this species undergoes a buffer-dependent self-assembly process: nanometer-scale globular materials were formed when these components were reacted in unbuffered H{sub 2}O, while millimeter-scale, rod-like materials were prepared when the reaction was performed in phosphate buffer (20 mM, pH 7). It is suggested that assembly of the globular material is dictated by the conformational properties of the coiled-coil forming AQ-Pal14 peptide, whereas that of the rod-like material involves interactions between Co-PPIX and phosphate ion.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE SC OFFICE OF SCIENCE (SC)
OSTI Identifier:
1042177
Report Number(s):
BNL-97855-2012-JA
TRN: US201212%%588
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
Biomacromolecules
Additional Journal Information:
Journal Volume: 11; Journal Issue: 10
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; BUFFERS; DESIGN; PEPTIDES; PHOSPHATES; POLYPEPTIDES; PORPHYRINS; PROTOPORPHYRINS; RESIDUES

Citation Formats

Zaytsev, D, Xie, F, Mukherjee, M, Bludin, A, Demeler, B, Breece, R, Tierney, D, and Ogawa, M. Nanometer to Millimeter Scale Peptide-Porphyrin Materials. United States: N. p., 2011. Web.
Zaytsev, D, Xie, F, Mukherjee, M, Bludin, A, Demeler, B, Breece, R, Tierney, D, & Ogawa, M. Nanometer to Millimeter Scale Peptide-Porphyrin Materials. United States.
Zaytsev, D, Xie, F, Mukherjee, M, Bludin, A, Demeler, B, Breece, R, Tierney, D, and Ogawa, M. 2011. "Nanometer to Millimeter Scale Peptide-Porphyrin Materials". United States.
@article{osti_1042177,
title = {Nanometer to Millimeter Scale Peptide-Porphyrin Materials},
author = {Zaytsev, D and Xie, F and Mukherjee, M and Bludin, A and Demeler, B and Breece, R and Tierney, D and Ogawa, M},
abstractNote = {AQ-Pal14 is a 30-residue polypeptide that was designed to form an {alpha}-helical coiled coil that contains a metal-binding 4-pyridylalanine residue on its solvent-exposed surface. However, characterization of this peptide shows that it exists as a three-stranded coiled coil, not a two-stranded one as predicted from its design. Reaction with cobalt(III) protoporphyrin IX (Co-PPIX) produces a six-coordinate Co-PPIX(AQ-Pal14){sub 2} species that creates two coiled-coil oligomerization domains coordinated to opposite faces of the porphyrin ring. It is found that this species undergoes a buffer-dependent self-assembly process: nanometer-scale globular materials were formed when these components were reacted in unbuffered H{sub 2}O, while millimeter-scale, rod-like materials were prepared when the reaction was performed in phosphate buffer (20 mM, pH 7). It is suggested that assembly of the globular material is dictated by the conformational properties of the coiled-coil forming AQ-Pal14 peptide, whereas that of the rod-like material involves interactions between Co-PPIX and phosphate ion.},
doi = {},
url = {https://www.osti.gov/biblio/1042177}, journal = {Biomacromolecules},
number = 10,
volume = 11,
place = {United States},
year = {2011},
month = {12}
}