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Title: Extensive gene flow over Europe and possible speciation

Abstract

Biogeographical patterns and large-scale genetic structure have been little studied in ectomycorrhizal (EM) fungi, despite the ecological and economic importance of EM symbioses. We coupled population genetics and phylogenetic approaches to understand spatial structure in fungal populations on a continental scale. Using nine microsatellite markers, we characterized gene flow among 16 populations of the widespread EM basidiomycete Laccaria amethystina over Europe (i.e. over 2900 km). We also widened our scope to two additional populations from Japan (104 km away) and compared them with European populations through microsatellite markers and multilocus phylogenies, using three nuclear genes (NAR, G6PD and ribosomal DNA) and two mitochondrial ribosomal genes. European L. amethystina populations displayed limited differentiation (average FST = 0.041) and very weak isolation by distance (IBD). This panmictic European pattern may result from effective aerial dispersal of spores, high genetic diversity in populations and mutualistic interactions with multiple hosts that all facilitate migration. The multilocus phylogeny based on nuclear genes confirmed that Japanese and European specimens were closely related but clustered on a geographical basis. By using microsatellite markers, we found that Japanese populations were strongly differentiated from the European populations (FST = 0.416), more than expected by extrapolating the European pattern ofmore » IBD. Population structure analyses clearly separated the populations into two clusters, i.e. European and Japanese clusters. We discuss the possibility of IBD in a continuous population (considering some evidence for a ring species over the Northern Hemisphere) vs. an allopatric speciation over Eurasia, making L. amethystina a promising model of intercontinental species for future studies.« less

Authors:
 [1];  [2];  [1];  [3];  [1];  [4];  [5];  [1]
  1. Centre d’Ecologie Fonctionnelle et Evolutive Montpellier, France
  2. Department of Natural Environmental Studies, The University of Tokyo, Japan
  3. ORNL
  4. University of Tartu, Estonia
  5. INRA, Nancy, France
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1041485
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Molecular Ecology
Additional Journal Information:
Journal Volume: 21; Journal Issue: 2; Journal ID: ISSN 0962-1083
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; DNA; ECONOMICS; EUROPE; FUNGI; GENES; GENETICS; NORTHERN HEMISPHERE; SPORES

Citation Formats

VINCENOT, Dr. LUCIE, NARA, Dr. KAZUHIDE, STHULTZ, CHRISTOPHER, Labbe, Jessy L, DUBOIS, MARIE-PIERRE, TEDERSOO, LEHO, Martin, Francis, and SELOSSE, Dr. MARC-ANDRE. Extensive gene flow over Europe and possible speciation. United States: N. p., 2012. Web. doi:10.1111/j.1365-294X.2011.05392.x.
VINCENOT, Dr. LUCIE, NARA, Dr. KAZUHIDE, STHULTZ, CHRISTOPHER, Labbe, Jessy L, DUBOIS, MARIE-PIERRE, TEDERSOO, LEHO, Martin, Francis, & SELOSSE, Dr. MARC-ANDRE. Extensive gene flow over Europe and possible speciation. United States. https://doi.org/10.1111/j.1365-294X.2011.05392.x
VINCENOT, Dr. LUCIE, NARA, Dr. KAZUHIDE, STHULTZ, CHRISTOPHER, Labbe, Jessy L, DUBOIS, MARIE-PIERRE, TEDERSOO, LEHO, Martin, Francis, and SELOSSE, Dr. MARC-ANDRE. 2012. "Extensive gene flow over Europe and possible speciation". United States. https://doi.org/10.1111/j.1365-294X.2011.05392.x.
@article{osti_1041485,
title = {Extensive gene flow over Europe and possible speciation},
author = {VINCENOT, Dr. LUCIE and NARA, Dr. KAZUHIDE and STHULTZ, CHRISTOPHER and Labbe, Jessy L and DUBOIS, MARIE-PIERRE and TEDERSOO, LEHO and Martin, Francis and SELOSSE, Dr. MARC-ANDRE},
abstractNote = {Biogeographical patterns and large-scale genetic structure have been little studied in ectomycorrhizal (EM) fungi, despite the ecological and economic importance of EM symbioses. We coupled population genetics and phylogenetic approaches to understand spatial structure in fungal populations on a continental scale. Using nine microsatellite markers, we characterized gene flow among 16 populations of the widespread EM basidiomycete Laccaria amethystina over Europe (i.e. over 2900 km). We also widened our scope to two additional populations from Japan (104 km away) and compared them with European populations through microsatellite markers and multilocus phylogenies, using three nuclear genes (NAR, G6PD and ribosomal DNA) and two mitochondrial ribosomal genes. European L. amethystina populations displayed limited differentiation (average FST = 0.041) and very weak isolation by distance (IBD). This panmictic European pattern may result from effective aerial dispersal of spores, high genetic diversity in populations and mutualistic interactions with multiple hosts that all facilitate migration. The multilocus phylogeny based on nuclear genes confirmed that Japanese and European specimens were closely related but clustered on a geographical basis. By using microsatellite markers, we found that Japanese populations were strongly differentiated from the European populations (FST = 0.416), more than expected by extrapolating the European pattern of IBD. Population structure analyses clearly separated the populations into two clusters, i.e. European and Japanese clusters. We discuss the possibility of IBD in a continuous population (considering some evidence for a ring species over the Northern Hemisphere) vs. an allopatric speciation over Eurasia, making L. amethystina a promising model of intercontinental species for future studies.},
doi = {10.1111/j.1365-294X.2011.05392.x},
url = {https://www.osti.gov/biblio/1041485}, journal = {Molecular Ecology},
issn = {0962-1083},
number = 2,
volume = 21,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2012},
month = {Sun Jan 01 00:00:00 EST 2012}
}