Structural Basis for the Rescue of Stalled Ribosomes: Structure of YaeJ Bound to the Ribosome
Abstract
In bacteria, the hybrid transfer-messenger RNA (tmRNA) rescues ribosomes stalled on defective messenger RNAs (mRNAs). However, certain gram-negative bacteria have evolved proteins that are capable of rescuing stalled ribosomes in a tmRNA-independent manner. Here, we report a 3.2 angstrom-resolution crystal structure of the rescue factor YaeJ bound to the Thermus thermophilus 70S ribosome in complex with the initiator tRNA{sub i}{sup fMet} and a short mRNA. The structure reveals that the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes by binding in the mRNA entry channel downstream of the A site between the head and shoulder of the 30S subunit. This allows the N-terminal globular domain to sample different conformations, so that its conserved GGQ motif is optimally positioned to catalyze the hydrolysis of peptidyl-tRNA. This structure gives insights into the mechanism of YaeJ function and provides a basis for understanding how it rescues stalled ribosomes.
- Authors:
-
- Yale
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- National Institutes of Health (NIH)
- OSTI Identifier:
- 1039065
- Resource Type:
- Journal Article
- Journal Name:
- Science
- Additional Journal Information:
- Journal Volume: 335; Journal Issue: 03, 2012; Journal ID: ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BACTERIA; CRYSTAL STRUCTURE; HYDROLYSIS; PROTEINS; RIBOSOMES; RNA; SENSORS
Citation Formats
Gagnon, Matthieu G, Seetharaman, Sai V, Bulkley, David, and Steitz, Thomas A. Structural Basis for the Rescue of Stalled Ribosomes: Structure of YaeJ Bound to the Ribosome. United States: N. p., 2012.
Web. doi:10.1126/science.1217443.
Gagnon, Matthieu G, Seetharaman, Sai V, Bulkley, David, & Steitz, Thomas A. Structural Basis for the Rescue of Stalled Ribosomes: Structure of YaeJ Bound to the Ribosome. United States. https://doi.org/10.1126/science.1217443
Gagnon, Matthieu G, Seetharaman, Sai V, Bulkley, David, and Steitz, Thomas A. Tue .
"Structural Basis for the Rescue of Stalled Ribosomes: Structure of YaeJ Bound to the Ribosome". United States. https://doi.org/10.1126/science.1217443.
@article{osti_1039065,
title = {Structural Basis for the Rescue of Stalled Ribosomes: Structure of YaeJ Bound to the Ribosome},
author = {Gagnon, Matthieu G and Seetharaman, Sai V and Bulkley, David and Steitz, Thomas A},
abstractNote = {In bacteria, the hybrid transfer-messenger RNA (tmRNA) rescues ribosomes stalled on defective messenger RNAs (mRNAs). However, certain gram-negative bacteria have evolved proteins that are capable of rescuing stalled ribosomes in a tmRNA-independent manner. Here, we report a 3.2 angstrom-resolution crystal structure of the rescue factor YaeJ bound to the Thermus thermophilus 70S ribosome in complex with the initiator tRNA{sub i}{sup fMet} and a short mRNA. The structure reveals that the C-terminal tail of YaeJ functions as a sensor to discriminate between stalled and actively translating ribosomes by binding in the mRNA entry channel downstream of the A site between the head and shoulder of the 30S subunit. This allows the N-terminal globular domain to sample different conformations, so that its conserved GGQ motif is optimally positioned to catalyze the hydrolysis of peptidyl-tRNA. This structure gives insights into the mechanism of YaeJ function and provides a basis for understanding how it rescues stalled ribosomes.},
doi = {10.1126/science.1217443},
url = {https://www.osti.gov/biblio/1039065},
journal = {Science},
issn = {0036-8075},
number = 03, 2012,
volume = 335,
place = {United States},
year = {2012},
month = {6}
}