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Title: Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals

Abstract

The recent analysis of the first successful RV144 vaccine trial revealed that a high titer of plasma anti-V2 antibodies (Abs) correlated with a decreased risk of HIV-1 infection in vaccine recipients. To understand the mechanism of immune correlates, we studied seven anti-V2 monoclonal Abs (mAbs) developed from HIV-1 infected individuals. The V2 mAbs target conserved epitopes, including the binding site for {alpha}4{beta}7 integrin, and are broadly cross-reactive with various gp120 proteins. Preferential usage of the VH1-69 gene by V2 mAbs may depend on selection by the same antigenic structure. Six of seven V2 mAbs weakly neutralized four to eight of the 41 pseudoviruses tested and resistance to neutralization was correlated with longer V2 domains. The data suggest the presence of shared, conserved structural elements in the V2 loop, and these can be used in the design of vaccine immunogens inducing broadly reactive Abs with anti-viral activities.

Authors:
; ; ; ; ; ; ; ; ; ; ; ;  [1]
  1. Harvard-Med
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
OTHERNIHOTHER U.S. GOVERNMENT
OSTI Identifier:
1038605
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 427; Journal Issue: (2) ; 06, 2012; Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; AIDS VIRUS; ANTIBODIES; DESIGN; FUNCTIONALS; GENES; MONOCLONAL ANTIBODIES; PLASMA; PROTEINS; TARGETS; VACCINES

Citation Formats

Gorny, Miroslaw K, Pan, Ruimin, Williams, Constance, Wang, Xiao-Hong, Volsky, Barbara, O,, Neal, Timothy, Spurrier, Brett, Sampson, Jared M, Li, Liuzhe, Seaman, Michael S, Kong, Xiang-Peng, Zolla-Pazner, Susan, VA), and NYUSM). Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals. United States: N. p., 2012. Web. doi:10.1016/j.virol.2012.02.003.
Gorny, Miroslaw K, Pan, Ruimin, Williams, Constance, Wang, Xiao-Hong, Volsky, Barbara, O,, Neal, Timothy, Spurrier, Brett, Sampson, Jared M, Li, Liuzhe, Seaman, Michael S, Kong, Xiang-Peng, Zolla-Pazner, Susan, VA), & NYUSM). Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals. United States. doi:10.1016/j.virol.2012.02.003.
Gorny, Miroslaw K, Pan, Ruimin, Williams, Constance, Wang, Xiao-Hong, Volsky, Barbara, O,, Neal, Timothy, Spurrier, Brett, Sampson, Jared M, Li, Liuzhe, Seaman, Michael S, Kong, Xiang-Peng, Zolla-Pazner, Susan, VA), and NYUSM). Fri . "Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals". United States. doi:10.1016/j.virol.2012.02.003.
@article{osti_1038605,
title = {Functional and immunochemical cross-reactivity of V2-specific monoclonal antibodies from HIV-1-infected individuals},
author = {Gorny, Miroslaw K and Pan, Ruimin and Williams, Constance and Wang, Xiao-Hong and Volsky, Barbara and O, and Neal, Timothy and Spurrier, Brett and Sampson, Jared M and Li, Liuzhe and Seaman, Michael S and Kong, Xiang-Peng and Zolla-Pazner, Susan and VA) and NYUSM)},
abstractNote = {The recent analysis of the first successful RV144 vaccine trial revealed that a high titer of plasma anti-V2 antibodies (Abs) correlated with a decreased risk of HIV-1 infection in vaccine recipients. To understand the mechanism of immune correlates, we studied seven anti-V2 monoclonal Abs (mAbs) developed from HIV-1 infected individuals. The V2 mAbs target conserved epitopes, including the binding site for {alpha}4{beta}7 integrin, and are broadly cross-reactive with various gp120 proteins. Preferential usage of the VH1-69 gene by V2 mAbs may depend on selection by the same antigenic structure. Six of seven V2 mAbs weakly neutralized four to eight of the 41 pseudoviruses tested and resistance to neutralization was correlated with longer V2 domains. The data suggest the presence of shared, conserved structural elements in the V2 loop, and these can be used in the design of vaccine immunogens inducing broadly reactive Abs with anti-viral activities.},
doi = {10.1016/j.virol.2012.02.003},
journal = {Virology},
issn = {0042-6822},
number = (2) ; 06, 2012,
volume = 427,
place = {United States},
year = {2012},
month = {5}
}