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Title: Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques

Abstract

Many human microbial infectious diseases including dental caries are polymicrobial in nature and how these complex multi-species communities evolve from a healthy to a diseased state is not well understood. Although many health- or disease-associated oral microbes have been characterized in vitro, their physiology in vivo in the presence of the complex oral microbiome is difficult to determine with current approaches. In addition, about half of these oral species remain uncultivated to date and little is known except their 16S rRNA sequence. Lacking culture-based physiological analyses, the functional roles of uncultivated microorganisms will remain enigmatic despite their apparent disease correlation. To start addressing these knowledge gaps, we applied a novel combination of in vivo Magnetic Resonance Spectroscopy (MRS) with RNA and DNA based Stable Isotope Probing (SIP) to oral plaque communities from healthy children for temporal monitoring of carbohydrate utilization, organic acid production and identification of metabolically active and inactive bacterial species.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1036913
Report Number(s):
PNNL-SA-83915
25694; TRN: US201206%%660
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
PLoS One, 7(3):Article No. e32219
Additional Journal Information:
Journal Volume: 7; Journal Issue: 3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; CARBOHYDRATES; CARIES; CHILDREN; COMMUNITIES; DISEASES; DNA; FUNCTIONALS; IN VITRO; IN VIVO; INFECTIOUS DISEASES; MAGNETIC RESONANCE; MICROORGANISMS; MONITORING; ORGANIC ACIDS; PHYSIOLOGY; PRODUCTION; RNA; SPECTROSCOPY; STABLE ISOTOPES; oral communities; polymicrobial; microbiome; Environmental Molecular Sciences Laboratory

Citation Formats

McLean, Jeffrey S., Fansler, Sarah J., Majors, Paul D., Mcateer, Kathleen, Allen, Lisa Z., Shirtliff, Mark E., Lux, Renate, and Shi, Wenyuan. Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques. United States: N. p., 2012. Web. doi:10.1371/journal.pone.0032219.
McLean, Jeffrey S., Fansler, Sarah J., Majors, Paul D., Mcateer, Kathleen, Allen, Lisa Z., Shirtliff, Mark E., Lux, Renate, & Shi, Wenyuan. Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques. United States. doi:10.1371/journal.pone.0032219.
McLean, Jeffrey S., Fansler, Sarah J., Majors, Paul D., Mcateer, Kathleen, Allen, Lisa Z., Shirtliff, Mark E., Lux, Renate, and Shi, Wenyuan. Mon . "Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques". United States. doi:10.1371/journal.pone.0032219.
@article{osti_1036913,
title = {Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques},
author = {McLean, Jeffrey S. and Fansler, Sarah J. and Majors, Paul D. and Mcateer, Kathleen and Allen, Lisa Z. and Shirtliff, Mark E. and Lux, Renate and Shi, Wenyuan},
abstractNote = {Many human microbial infectious diseases including dental caries are polymicrobial in nature and how these complex multi-species communities evolve from a healthy to a diseased state is not well understood. Although many health- or disease-associated oral microbes have been characterized in vitro, their physiology in vivo in the presence of the complex oral microbiome is difficult to determine with current approaches. In addition, about half of these oral species remain uncultivated to date and little is known except their 16S rRNA sequence. Lacking culture-based physiological analyses, the functional roles of uncultivated microorganisms will remain enigmatic despite their apparent disease correlation. To start addressing these knowledge gaps, we applied a novel combination of in vivo Magnetic Resonance Spectroscopy (MRS) with RNA and DNA based Stable Isotope Probing (SIP) to oral plaque communities from healthy children for temporal monitoring of carbohydrate utilization, organic acid production and identification of metabolically active and inactive bacterial species.},
doi = {10.1371/journal.pone.0032219},
journal = {PLoS One, 7(3):Article No. e32219},
number = 3,
volume = 7,
place = {United States},
year = {2012},
month = {3}
}