Engineered microorganisms capable of producing target compounds under anaerobic conditions

Buelter, Thomas; Meinhold, Peter; Feldman, Reid M. Renny; Hawkins, Andrew C; Urano, Jun; Bastian, Sabine; Arnold, Frances

Title: Engineered microorganisms capable of producing target compounds under anaerobic conditions

Patent · Tue Jan 17 00:00:00 EST 2012

OSTI ID:1034914

Buelter, Thomas ^[1]; Meinhold, Peter ^[1]; Feldman, Reid M. Renny ^[2]; Hawkins, Andrew C ^[3]; Urano, Jun ^[4]; Bastian, Sabine ^[5]; Arnold, Frances ^[6]

Denver, CO
San Francisco, CA
Parker, CO
Irvine, CA
Pasadena, CA
La Canada, CA

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

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Research Organization:: Gevo, Inc. (Englewood, CO)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-07ER84893

Assignee:: Gevo, Inc. (Englewood, CO); California Institute of Technology (Pasadena, CA)

Patent Number(s):: 8,097,440

Application Number:: 13/269,937

OSTI ID:: 1034914

Country of Publication:: United States

Language:: English

References (27)

Reversal of the Nucleotide Specificity of Ketol Acid Reductoisomerase by Site-Directed Mutagenesis Identifies the NADPH Binding Site1 Rane, Madhavi J.; Calvo, K. C. Archives of Biochemistry and Biophysics, Vol. 338, Issue 1 https://doi.org/10.1006/abbi.1996.9802	journal	February 1997
Expression of protein engineered NADP+-dependent xylitol dehydrogenase increases ethanol production from xylose in recombinant Saccharomyces cerevisiae Matsushika, Akinori; Watanabe, Seiya; Kodaki, Tsutomu Applied Microbiology and Biotechnology, Vol. 81, Issue 2 https://doi.org/10.1007/s00253-008-1649-1	journal	November 2008
Manipulation of malic enzyme in Saccharomyces cerevisiae for increasing NADPH production capacity aerobically in different cellular compartments Moreira dos Santos, Margarida; Raghevendran, Vijayendran; Kötter, Peter Metabolic Engineering, Vol. 6, Issue 4 https://doi.org/10.1016/j.ymben.2004.06.002	journal	October 2004
Metabolic Engineering of Saccharomyces cerevisiae Ostergaard, S.; Olsson, L.; Nielsen, J. Microbiology and Molecular Biology Reviews, Vol. 64, Issue 1 https://doi.org/10.1128/MMBR.64.1.34-50.2000	journal	March 2000
Redesign of the coenzyme specificity of a dehydrogenase by protein engineering Scrutton, Nigel S.; Berry, Alan; Perham, Richard N. Nature, Vol. 343, Issue 6253 https://doi.org/10.1038/343038a0	journal	January 1990
Cofactor Regeneration by a Soluble Pyridine Nucleotide Transhydrogenase for Biological Production of Hydromorphone Boonstra, B.; Rathbone, D. A.; French, C. E. Applied and Environmental Microbiology, Vol. 66, Issue 12 https://doi.org/10.1128/AEM.66.12.5161-5166.2000	journal	December 2000
The expression of aPichia stipitis xylose reductase mutant with higherKM for NADPH increases ethanol production from xylose in recombinantSaccharomyces cerevisiae Jeppsson, Marie; Bengtsson, Oskar; Franke, Katja Biotechnology and Bioengineering, Vol. 93, Issue 4 https://doi.org/10.1002/bit.20737	journal	January 2006
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein-engineered NADH-preferring xylose reductase from Pichia stipitis Watanabe, S.; Abu Saleh, A.; Pack, S. P. Microbiology, Vol. 153, Issue 9 https://doi.org/10.1099/mic.0.2007/007856-0	journal	September 2007
Improved synthesis of chiral alcohols with Escherichia coli cells co-expressing pyridine nucleotide transhydrogenase, NADP+-dependent alcohol dehydrogenase and NAD+-dependent formate dehydrogenase Weckbecker, Andrea; Hummel, Werner Biotechnology Letters, Vol. 26, Issue 22 https://doi.org/10.1007/s10529-004-3746-2	journal	November 2004
Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein. Benyajati, C.; Place, A. R.; Powers, D. A. Proceedings of the National Academy of Sciences, Vol. 78, Issue 5 https://doi.org/10.1073/pnas.78.5.2717	journal	May 1981
Complete Reversal of Coenzyme Specificity by Concerted Mutation of Three Consecutive Residues in Alcohol Dehydrogenase Rosell, Albert; Valencia, Eva; Ochoa, Wendy F. Journal of Biological Chemistry, Vol. 278, Issue 42 https://doi.org/10.1074/jbc.M307384200	journal	August 2003
Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA Canonaco, Fabrizio; Hess, Tracy A.; Heri, Sylvia FEMS Microbiology Letters, Vol. 204, Issue 2 https://doi.org/10.1111/j.1574-6968.2001.tb10892.x	journal	November 2001
Novel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia coli Echave, Pedro; Tamarit, Jordi; Cabiscol, Elisa Journal of Biological Chemistry, Vol. 278, Issue 32 https://doi.org/10.1074/jbc.M304351200	journal	June 2003
Ethanol production from xylose by recombinant Saccharomyces cerevisiae expressing protein engineered NADP+-dependent xylitol dehydrogenase Watanabe, Seiya; Saleh, Ahmed Abu; Pack, Seung Pil Journal of Biotechnology, Vol. 130, Issue 3 https://doi.org/10.1016/j.jbiotec.2007.04.019	journal	June 2007
Expression of a cytoplasmic transhydrogenase inSaccharomyces cerevisiae results in formation of 2-oxoglutarate due to depletion of the NADPH pool Nissen, Torben L.; Anderlund, Mikael; Nielsen, Jens Yeast, Vol. 18, Issue 1, p. 19-32 https://doi.org/10.1002/1097-0061(200101)18:1<19::AID-YEA650>3.0.CO;2-5	journal	January 2000
Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase Matsushika, Akinori; Watanabe, Seiya; Kodaki, Tsutomu Journal of Bioscience and Bioengineering, Vol. 105, Issue 3 https://doi.org/10.1263/jbb.105.296	journal	March 2008
Metabolic engineering of Escherichia coli for 1-butanol production Atsumi, Shota; Cann, Anthony F.; Connor, Michael R. Metabolic Engineering, Vol. 10, Issue 6, p. 305-311 https://doi.org/10.1016/j.ymben.2007.08.003	journal	November 2008
Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae Petschacher, Barbara; Nidetzky, Bernd Microbial Cell Factories, Vol. 7, Issue 1 https://doi.org/10.1186/1475-2859-7-9	journal	January 2008
Metabolic engineering for advanced biofuels production from Escherichia coli Atsumi, Shota; Liao, James C. Current Opinion in Biotechnology, Vol. 19, Issue 5, p. 414-419 https://doi.org/10.1016/j.copbio.2008.08.008	journal	October 2008
The Soluble and Membrane-bound Transhydrogenases UdhA and PntAB Have Divergent Functions in NADPH Metabolism of Escherichia coli Sauer, Uwe; Canonaco, Fabrizio; Heri, Sylvia Journal of Biological Chemistry, Vol. 279, Issue 8, p. 6613-6619 https://doi.org/10.1074/jbc.M311657200	journal	December 2003
Metabolic engineering of Escherichia coli for 1-butanol and 1-propanol production via the keto-acid pathways Shen, C. R.; Liao, J. C. Metabolic Engineering, Vol. 10, Issue 6 https://doi.org/10.1016/j.ymben.2008.08.001	journal	November 2008
Expression of the Escherichia coli pntA andpntB Genes, Encoding Nicotinamide Nucleotide Transhydrogenase, in Saccharomyces cerevisiae and Its Effect on Product Formation during Anaerobic Glucose Fermentation Anderlund, Mikael; Nissen, Torben L.; Nielsen, Jens Applied and Environmental Microbiology, Vol. 65, Issue 6 https://doi.org/10.1128/AEM.65.6.2333-2340.1999	journal	January 1999
Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels Lee, Sung Kuk; Chou, Howard; Ham, Timothy S. Current Opinion in Biotechnology, Vol. 19, Issue 6, p. 556-563 https://doi.org/10.1016/j.copbio.2008.10.014	journal	December 2008
Replacing Escherichia coli NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with a NADP-dependent enzyme from Clostridium acetobutylicum facilitates NADPH dependent pathways Martínez, Irene; Zhu, Jiangfeng; Lin, Henry Metabolic Engineering, Vol. 10, Issue 6 https://doi.org/10.1016/j.ymben.2008.09.001	journal	November 2008
Expression of Azotobacter vinelandii soluble transhydrogenase perturbs xylose reductase-mediated conversion of xylose to xylitol by recombinant Saccharomyces cerevisiae Jeun, Young-Sok; Kim, Myoung-Dong; Park, Yong-Cheol Journal of Molecular Catalysis B: Enzymatic, Vol. 26, Issue 3-6 https://doi.org/10.1016/j.molcatb.2003.07.003	journal	December 2003
Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes Atsumi, Shota; Wu, Tung-Yun; Eckl, Eva-Maria Applied Microbiology and Biotechnology, Vol. 85, Issue 3 https://doi.org/10.1007/s00253-009-2085-6	journal	July 2009
In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production Bro, Christoffer; Regenberg, Birgitte; Forster, Jochen Metabolic Engineering, Vol. 8, Issue 2, p. 102-111 https://doi.org/10.1016/j.ymben.2005.09.007	journal	November 2005

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