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Title: Enzyme activity in dialkyl phosphate ionic liquids

Abstract

The activity of four metagenomic enzymes and an enzyme cloned from the straw mushroom, Volvariellavolvacea were studied in the following ionic liquids, 1,3-dimethylimidazolium dimethyl phosphate, [mmim][dmp], 1-ethyl-3-methylimidazolium dimethyl phosphate, [emim][dmp], 1-ethyl-3-methylimidazolium diethyl phosphate, [emim][dep] and 1-ethyl-3-methylimidazolium acetate, [emim][OAc]. Activity was determined by analyzing the hydrolysis of para-nitrobenzene carbohydrate derivatives. In general, the enzymes were most active in the dimethyl phosphate ionic liquids, followed by acetate. Generally speaking, activity decreased sharply for concentrations of [emim][dep] above 10% v/v, while the other ionic liquids showed less impact on activity up to 20% v/v.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
BROOKHAVEN NATIONAL LABORATORY (BNL)
Sponsoring Org.:
LABORATORY-DIRECTED RESEARCH AND DEVELOPMENT
OSTI Identifier:
1034739
Report Number(s):
BNL-96652-2011-JA
Journal ID: ISSN 0960-8524; BIRTEB; R&D Project: 08-069; BOW-139; YN0100000; TRN: US201204%%158
DOE Contract Number:  
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bioresource Technology; Journal Volume: 102; Journal Issue: 24
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; CARBOHYDRATES; CELLULASE; ENZYMATIC HYDROLYSIS; ENZYME ACTIVITY; ENZYMES; HYDROLYSIS; MOLTEN SALTS; PHOSPHATES; Ionic liquid; Cellulases; Enzyme activity; Enzymatic hydrolysis

Citation Formats

Thomas, M.F., Dunn, J., Li, L.-L., Handley-Pendleton, J. M., van der lelie, D., and Wishart, J. F. Enzyme activity in dialkyl phosphate ionic liquids. United States: N. p., 2011. Web. doi:10.1016/j.biortech.2011.09.069.
Thomas, M.F., Dunn, J., Li, L.-L., Handley-Pendleton, J. M., van der lelie, D., & Wishart, J. F. Enzyme activity in dialkyl phosphate ionic liquids. United States. doi:10.1016/j.biortech.2011.09.069.
Thomas, M.F., Dunn, J., Li, L.-L., Handley-Pendleton, J. M., van der lelie, D., and Wishart, J. F. Thu . "Enzyme activity in dialkyl phosphate ionic liquids". United States. doi:10.1016/j.biortech.2011.09.069.
@article{osti_1034739,
title = {Enzyme activity in dialkyl phosphate ionic liquids},
author = {Thomas, M.F. and Dunn, J. and Li, L.-L. and Handley-Pendleton, J. M. and van der lelie, D. and Wishart, J. F.},
abstractNote = {The activity of four metagenomic enzymes and an enzyme cloned from the straw mushroom, Volvariellavolvacea were studied in the following ionic liquids, 1,3-dimethylimidazolium dimethyl phosphate, [mmim][dmp], 1-ethyl-3-methylimidazolium dimethyl phosphate, [emim][dmp], 1-ethyl-3-methylimidazolium diethyl phosphate, [emim][dep] and 1-ethyl-3-methylimidazolium acetate, [emim][OAc]. Activity was determined by analyzing the hydrolysis of para-nitrobenzene carbohydrate derivatives. In general, the enzymes were most active in the dimethyl phosphate ionic liquids, followed by acetate. Generally speaking, activity decreased sharply for concentrations of [emim][dep] above 10% v/v, while the other ionic liquids showed less impact on activity up to 20% v/v.},
doi = {10.1016/j.biortech.2011.09.069},
journal = {Bioresource Technology},
number = 24,
volume = 102,
place = {United States},
year = {Thu Dec 01 00:00:00 EST 2011},
month = {Thu Dec 01 00:00:00 EST 2011}
}