Method and apparatus for sustaining viability of biological cells on a substrate

McKnight, Timothy E; Melechko, Anatoli V; Simpson, Michael L

Method and apparatus for sustaining viability of biological cells on a substrate

Patent · 13 December 2011

OSTI ID:1034300

McKnight, Timothy E ^[1]; Melechko, Anatoli V ^[2]; Simpson, Michael L ^[3]

Greenback, TN
Oak Ridge, TN
Knoxville, TN

A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 8,076,124

Application Number:: 11/624,040

OSTI ID:: 1034300

Country of Publication:: United States

Language:: English

References (46)

Alignment mechanism of carbon nanofibers produced by plasma-enhanced chemical-vapor deposition Merkulov, Vladimir I.; Melechko, Anatoli V.; Guillorn, Michael A. Applied Physics Letters, Vol. 79, Issue 18 https://doi.org/10.1063/1.1415411	journal	October 2001
The use of Chinese hamster ovary cells to quantify specific locus mutation and to determine mutagenicity of chemicals: A report of the GENE-TOX program Hsie, A. W.; Casciano, D. A.; Couch, D. B. Mutation Research/Reviews in Genetic Toxicology, Vol. 86, Issue 2, p. 193-214 https://doi.org/10.1016/0165-1110(81)90024-5	journal	March 1981
Fabrication of Poly(ethylene glycol) Hydrogel Microstructures Using Photolithography Revzin, Alexander; Russell, Ryan J.; Yadavalli, Vamsi K. Langmuir, Vol. 17, Issue 18 https://doi.org/10.1021/la010075w	journal	September 2001
Nanotube Nanotweezers Kim, P. Science, Vol. 286, Issue 5447 https://doi.org/10.1126/science.286.5447.2148	journal	December 1999
Semiconductor Nanocrystals as Fluorescent Biological Labels Bruchez Jr., Marcel; Moronne, Mario Moronne; Gin, Peter Gin Science, Vol. 281, Issue 5385, p. 2013-2016 https://doi.org/10.1126/science.281.5385.2013	patent	September 1998
A molecular delivery system by using AFM and nanoneedle Han, Sung Woong; Nakamura, Chikashi; Obataya, Ikuo Biosensors and Bioelectronics, Vol. 20, Issue 10 https://doi.org/10.1016/j.bios.2004.08.023	journal	April 2005
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection Chan, Warren C. W.; Nie, Shuming Science, Vol. 281, Issue 5385, p. 2016-2018 https://doi.org/10.1126/science.281.5385.2016	journal	September 1998
A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes Knoblauch, Michael; Hibberd, Julian M.; Gray, John C. Nature Biotechnology, Vol. 17, Issue 9 https://doi.org/10.1038/12902	journal	September 1999
Microarrays of cells expressing defined cDNAs Ziauddin, Junaid; Sabatini, David M. Nature, Vol. 411, Issue 6833 https://doi.org/10.1038/35075114	journal	May 2001
Major limitations in the use of cationic liposomes for DNA delivery Filion, Mario C.; Phillips, Nigel C. International Journal of Pharmaceutics, Vol. 162, Issue 1-2, p. 159-170 https://doi.org/10.1016/S0378-5173(97)00423-7	journal	March 1998
Shaping carbon nanostructures by controlling the synthesis process Merkulov, Vladimir I.; Guillorn, Michael A.; Lowndes, Douglas H. Applied Physics Letters, Vol. 79, Issue 8 https://doi.org/10.1063/1.1395517	journal	August 2001
Engineered gene circuits Hasty, Jeff; McMillen, David; Collins, J. J. Nature, Vol. 420, Issue 6912 https://doi.org/10.1038/nature01257	journal	November 2002
The emerging field of nanotube biotechnology Martin, Charles R.; Kohli, Punit Nature Reviews Drug Discovery, Vol. 2, Issue 1 https://doi.org/10.1038/nrd988	journal	January 2003
Preparation of Nucleic Acid Functionalized Carbon Nanotube Arrays Nguyen, Cattien V.; Delzeit, Lance; Cassell, Alan M. Nano Letters, Vol. 2, Issue 10 https://doi.org/10.1021/nl025689f	journal	October 2002
Modelling cellular behaviour Endy, Drew; Brent, Roger Nature, Vol. 409, Issue 6818 https://doi.org/10.1038/35053181	journal	January 2001
Large-Scale Synthesis of Aligned Carbon Nanotubes Li, W. Z.; Xie, S. S.; Qian, L. X. Science, Vol. 274, Issue 5293 https://doi.org/10.1126/science.274.5293.1701	journal	December 1996
Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna Hamad-Schifferli, Kimberly; Schwartz, John J.; Santos, Aaron T. Nature, Vol. 415, Issue 6868 https://doi.org/10.1038/415152a	journal	January 2002
Activation of the NADPH oxidase in human fibroblasts by mechanical intrusion of a single cell with an ultramicroelectrode Arbault, S. Carcinogenesis, Vol. 18, Issue 3 https://doi.org/10.1093/carcin/18.3.569	journal	March 1997
Covalently functionalized nanotubes as nanometre- sized probes in chemistry and biology Wong, Stanislaus S.; Joselevich, Ernesto; Woolley, Adam T. Nature, Vol. 394, Issue 6688 https://doi.org/10.1038/27873	journal	July 1998
Field emission properties of different forms of carbon Merkulov, Vladimir I.; Lowndes, Douglas H.; Baylor, Larry R. Solid-State Electronics, Vol. 45, Issue 6, p. 949-956 https://doi.org/10.1016/S0038-1101(01)00036-3	journal	June 2001
Tracking Gene Expression after DNA Delivery Using Spatially Indexed Nanofiber Arrays McKnight, Timothy E.; Melechko, Anatoli V.; Hensley, Dale K. Nano Letters, Vol. 4, Issue 7 https://doi.org/10.1021/nl049504b	journal	July 2004
A synthetic oscillatory network of transcriptional regulators Elowitz, Michael B.; Leibler, Stanislas Nature, Vol. 403, Issue 6767 https://doi.org/10.1038/35002125	journal	January 2000
Carbon nanotube array-based biosensor Sotiropoulou, Sofia; Chaniotakis, Nikolas A. Analytical and Bioanalytical Chemistry, Vol. 375, Issue 1 https://doi.org/10.1007/s00216-002-1617-z	journal	January 2003
Combinatorial Synthesis of Genetic Networks Guet, C&a. ;l. C. Science, Vol. 296, Issue 5572 https://doi.org/10.1126/science.1067407	journal	May 2002
Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations Cong, B.; Liu, J.; Tanksley, S. D. Proceedings of the National Academy of Sciences, Vol. 99, Issue 21, p. 13606-13611 https://doi.org/10.1073/pnas.172520999	journal	October 2002
Synthesis of Large Arrays of Well-Aligned Carbon Nanotubes on Glass Ren, Z. F. Science, Vol. 282, Issue 5391 https://doi.org/10.1126/science.282.5391.1105	journal	November 1998
Individually addressable vertically aligned carbon nanofiber-based electrochemical probes Guillorn, M. A.; McKnight, T. E.; Melechko, A. Journal of Applied Physics, Vol. 91, Issue 6 https://doi.org/10.1063/1.1448671	journal	March 2002
DNA-functionalized single-walled carbon nanotubes Dwyer, Chris; Guthold, Martin; Falvo, Michael Nanotechnology, Vol. 13, Issue 5 https://doi.org/10.1088/0957-4484/13/5/311	journal	September 2002
Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells. Wightman, R. M.; Jankowski, J. A.; Kennedy, R. T. Proceedings of the National Academy of Sciences, Vol. 88, Issue 23 https://doi.org/10.1073/pnas.88.23.10754	journal	December 1991
Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness Han, SungWoong; Nakamura, Chikashi; Obataya, Ikuo Biochemical and Biophysical Research Communications, Vol. 332, Issue 3 https://doi.org/10.1016/j.bbrc.2005.04.059	journal	July 2005
Direct haplotyping of kilobase-size DNA using carbon nanotube probes Woolley, Adam T.; Guillemette, Chantal; Li Cheung, Chin Nature Biotechnology, Vol. 18, Issue 7 https://doi.org/10.1038/77760	journal	July 2000
Synthetic DNA delivery systems Luo, Dan; Saltzman, W. Mark Nature Biotechnology, Vol. 18, Issue 1 https://doi.org/10.1038/71889	journal	January 2000
Micromachined interfaces: new approaches in cell immunoisolation and biomolecular separation Desai, Tejal A.; Hansford, Derek J.; Ferrari, Mauro Biomolecular Engineering, Vol. 17, Issue 1, p. 23-36 https://doi.org/10.1016/S1389-0344(00)00063-0	journal	October 2000
Laterally Stabilized Complexes of DNA with Linear Reducible Polycations: Strategy for Triggered Intracellular Activation of DNA Delivery Vectors Oupický, David; Parker, Alan L.; Seymour, Leonard W. Journal of the American Chemical Society, Vol. 124, Issue 1 https://doi.org/10.1021/ja016440n	journal	January 2002
Whole-cell biocomputing Simpson, Michael L.; Sayler, Gary S.; Fleming, James T. Trends in Biotechnology, Vol. 19, Issue 8, p. 317-323 https://doi.org/10.1016/S0167-7799(01)01691-2	journal	August 2001
Microfabricated Microneedles for Gene and Drug Delivery McAllister, Devin V.; Allen, Mark G.; Prausnitz, Mark R. Annual Review of Biomedical Engineering, Vol. 2, Issue 1, p. 289-313 https://doi.org/10.1146/annurev.bioeng.2.1.289	journal	August 2000
Effects of spatial separation on the growth of vertically aligned carbon nanofibers produced by plasma-enhanced chemical vapor deposition Merkulov, Vladimir I.; Melechko, Anatoli V.; Guillorn, Michael A. Applied Physics Letters, Vol. 80, Issue 3 https://doi.org/10.1063/1.1433905	journal	January 2002
Fabrication and Characterization of Vertically Aligned Carbon Nanotubes on Silicon Substrates Using Porous Alumina Nanotemplates Hu, Wenchong; Yuan, Liming; Chen, Zhi Journal of Nanoscience and Nanotechnology, Vol. 2, Issue 2 https://doi.org/10.1166/jnn.2002.104	journal	April 2002
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles Dubertret, B. Science, Vol. 298, Issue 5599 https://doi.org/10.1126/science.1077194	journal	November 2002
Block and Graft Copolymers and Nanogel™ Copolymer Networks for DNA Delivery into Cell Lemieux, P.; Vinogradov, S.; Gebhart, C. Journal of Drug Targeting, Vol. 8, Issue 2 https://doi.org/10.3109/10611860008996855	journal	January 2000
Construction of a genetic toggle switch in Escherichia coli Gardner, Timothy S.; Cantor, Charles R.; Collins, James J. Nature, Vol. 403, Issue 6767 https://doi.org/10.1038/35002131	journal	January 2000
Patterned growth of individual and multiple vertically aligned carbon nanofibers Merkulov, V. I.; Lowndes, D. H.; Wei, Y. Y. Applied Physics Letters, Vol. 76, Issue 24 https://doi.org/10.1063/1.126705	journal	June 2000
Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection Cai, Hong; Cao, Xuni; Jiang, Ying Analytical and Bioanalytical Chemistry, Vol. 375, Issue 2 https://doi.org/10.1007/s00216-002-1652-9	journal	January 2003
Electrochemically induced adsorption of radio-labeled DNA on gold and HOPG substrates for STM investigations Brown, G. M.; Allison, D. P.; Warmack, R .J. Ultramicroscopy, Vol. 38, Issue 3-4, p. 253-264 https://doi.org/10.1016/0304-3991(91)90160-8	journal	December 1991
Field-emission studies of smooth and nanostructured carbon films Merkulov, Vladimir I.; Lowndes, Douglas H.; Baylor, Larry R. Applied Physics Letters, Vol. 75, Issue 9 https://doi.org/10.1063/1.124650	journal	August 1999
Structural and functional imaging with carbon nanotube AFM probes Hafner, J. Progress in Biophysics and Molecular Biology, Vol. 77, Issue 1 https://doi.org/10.1016/S0079-6107(01)00011-6	journal	January 2001

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