HPTLC Plate Blotting for Liquid Microjunction Surface Sampling Probe Mass Spectrometric Analysis of Analytes Separated on a Wettable Phase Plate

Journal Article · Sun Jan 01 00:00:00 EST 2012 · Rapid Communications in Mass Spectrometry

DOI:https://doi.org/10.1002/rcm.5299· OSTI ID:1032814

Walworth, Matthew J ^[1]; Stankovich, Joseph J ^[1]; Van Berkel, Gary J ^[1]; Schulz, Michael ^[2]; Minarik, susanne ^[2]

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1. ORNL
2. Merck Research Laboratories

A blotting method that transfers analytes separated on wettable HPTLC plates to a hydrophobic reversed-phase C8 HPLTC plate suitable for analysis with a liquid microjunction surface sampling probe electrospray ionization mass spectrometry system was described and demonstrated. The simple blotting procedure transfers the analyte from the wettable plate to the topmost surface of a rigidly backed, easy-to-mount hydrophobic substrate that already has been proven viable for analysis by this sampling probe/mass spectrometry system. The utility of the approach was demonstrated by the analysis of a four-component peptide mixture originally separated on a ProteoChrom HPTLC cellulose sheet and then blotted to the reversed phase HPTLC plate.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: Work for Others (WFO); USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1032814
• Journal Information: Rapid Communications in Mass Spectrometry, Vol. 26, Issue 1; ISSN 0951-4198
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CELLULOSE
IONIZATION
MASS SPECTROSCOPY
MIXTURES
PEPTIDES
PLATES
PROBES
SAMPLING
SPECTROSCOPY
SUBSTRATES
HPTLC
blotting
mass spectrometry
liquid microjunction surface sampling probe
electrospray ionization
hydrophobic reversed-phase
peptides