Highly thermostable fluorescent proteins

Patent · 29 November 2011

OSTI ID:1032633

Bradbury, Andrew M ^[1]; Waldo, Geoffrey S ^[1]; Kiss, Csaba ^[2]

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1. Santa Fe, NM
2. Los Alamos, NM

Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC52-06NA25396
• Assignee: Los Alamos National Security, LLC (Los Alamos, NM)
• Patent Number(s): 8,067,541
• Application Number: 13/023,421
• OSTI ID: 1032633
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES