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Title: Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance

Abstract

Biomass is one of the most abundant potential sustainable sources for fuel and material production, however to fully realize this potential an improved understanding of lignocellulosic recalcitrance must be developed. In an effort to appreciate the underlying phenotypic, biochemical and morphological properties associated with the reduced recalcitrance observed in tension stress-induced reaction wood, we report the increased enzymatic sugar yield and corresponding chemical and ultrastructural properties of Populus tension wood. Populus tremula x alba (PTA) was grown under tension and stem segments containing three different wood types: normal wood (NW), tension wood (TW) from the elongated stem side and opposite wood (OW) from the compressed stem side were collected. A variety of analytical techniques were used to describe changes occurring as a result of the tension stress-induced formation of a gelatinous cell wall layer (G-layer). For example, gel permeation chromatography (GPC) and 13C solid-state nuclear magnetic resonance (NMR) revealed that the molecular weight and crystallinity of cellulose in TW is greater than that of cellulose acquired from NW. Whole cell ionic liquid and other solid-state NMR analysis detailed the structure of lignin and hemicellulose in the samples, detecting the presence of variations in lignin and hemicellulose sub-units, linkages and semi-quantitativelymore » estimating the relative amounts of syringyl (S), guaiacyl (G) and p-hydroxybenzoate (PB) monolignol units. It was confirmed that TW displayed an increase in PB or H-like lignin and S to G ratio from 1.25 to 1.50 when compared to the NW sample. Scanning electron microscopy (SEM) and coherent anti-Stokes Raman scattering (CARS) were also used to evaluate the morphology and corresponding spatial distribution of the major lignocellulosic components. We found changes in a combination of cell wall properties appear to influence recalcitrance more than any single factor alone.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1029957
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Energy & Environmental Science
Additional Journal Information:
Journal Volume: 10.1039; Journal Issue: C1EE0207; Journal ID: ISSN 1754-5692
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; BIOMASS; CELL WALL; CELLULOSE; GEL PERMEATION CHROMATOGRAPHY; HEMICELLULOSE; LIGNIN; MOLECULAR WEIGHT; MOLTEN SALTS; MORPHOLOGY; NUCLEAR MAGNETIC RESONANCE; PRODUCTION; SACCHAROSE; SCANNING ELECTRON MICROSCOPY; SCATTERING; SPATIAL DISTRIBUTION; SUBSTRATES; WOOD

Citation Formats

Foston, Marcus B, Hubbell, Christopher A, Samuel, Reichel, Jung, Seung-Yong, Ding, Shi-You, Zeng, Yining, Jawdy, Sara, Sykes, Virginia R, Tuskan, Gerald A, Kalluri, Udaya C, and Ragauskas, Arthur J. Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance. United States: N. p., 2011. Web. doi:10.1039/c1ee02073k.
Foston, Marcus B, Hubbell, Christopher A, Samuel, Reichel, Jung, Seung-Yong, Ding, Shi-You, Zeng, Yining, Jawdy, Sara, Sykes, Virginia R, Tuskan, Gerald A, Kalluri, Udaya C, & Ragauskas, Arthur J. Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance. United States. doi:10.1039/c1ee02073k.
Foston, Marcus B, Hubbell, Christopher A, Samuel, Reichel, Jung, Seung-Yong, Ding, Shi-You, Zeng, Yining, Jawdy, Sara, Sykes, Virginia R, Tuskan, Gerald A, Kalluri, Udaya C, and Ragauskas, Arthur J. Sat . "Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance". United States. doi:10.1039/c1ee02073k.
@article{osti_1029957,
title = {Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance},
author = {Foston, Marcus B and Hubbell, Christopher A and Samuel, Reichel and Jung, Seung-Yong and Ding, Shi-You and Zeng, Yining and Jawdy, Sara and Sykes, Virginia R and Tuskan, Gerald A and Kalluri, Udaya C and Ragauskas, Arthur J},
abstractNote = {Biomass is one of the most abundant potential sustainable sources for fuel and material production, however to fully realize this potential an improved understanding of lignocellulosic recalcitrance must be developed. In an effort to appreciate the underlying phenotypic, biochemical and morphological properties associated with the reduced recalcitrance observed in tension stress-induced reaction wood, we report the increased enzymatic sugar yield and corresponding chemical and ultrastructural properties of Populus tension wood. Populus tremula x alba (PTA) was grown under tension and stem segments containing three different wood types: normal wood (NW), tension wood (TW) from the elongated stem side and opposite wood (OW) from the compressed stem side were collected. A variety of analytical techniques were used to describe changes occurring as a result of the tension stress-induced formation of a gelatinous cell wall layer (G-layer). For example, gel permeation chromatography (GPC) and 13C solid-state nuclear magnetic resonance (NMR) revealed that the molecular weight and crystallinity of cellulose in TW is greater than that of cellulose acquired from NW. Whole cell ionic liquid and other solid-state NMR analysis detailed the structure of lignin and hemicellulose in the samples, detecting the presence of variations in lignin and hemicellulose sub-units, linkages and semi-quantitatively estimating the relative amounts of syringyl (S), guaiacyl (G) and p-hydroxybenzoate (PB) monolignol units. It was confirmed that TW displayed an increase in PB or H-like lignin and S to G ratio from 1.25 to 1.50 when compared to the NW sample. Scanning electron microscopy (SEM) and coherent anti-Stokes Raman scattering (CARS) were also used to evaluate the morphology and corresponding spatial distribution of the major lignocellulosic components. We found changes in a combination of cell wall properties appear to influence recalcitrance more than any single factor alone.},
doi = {10.1039/c1ee02073k},
journal = {Energy & Environmental Science},
issn = {1754-5692},
number = C1EE0207,
volume = 10.1039,
place = {United States},
year = {2011},
month = {1}
}