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Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Patent ·
OSTI ID:1029285
 [1];  [2];  [3];  [4]
  1. San Francisco, CA
  2. Brookline, MA
  3. Cambridge, MA
  4. La Jolla, CA
+ Show Author Affiliations

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Research Organization:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,030,028
Application Number:
12/590,742
OSTI ID:
1029285
Country of Publication:
United States
Language:
English

References (32)

Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ journal August 1981
Exploring the Limits of Codon and Anticodon Size journal February 2002
Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion journal May 2003
Insertions in the anticodon loop of tRNA1Gln(sufG) and tRNALys promote quadruplet decoding of CAAA journal May 2002
Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression journal January 1996
Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement journal January 1999
Actions of the anticodon arm in translation on the phenotypes of RNA mutants journal November 1986
An expanded genetic code with a functional quadruplet codon journal May 2004
Generation of a Bacterium with a 21 Amino Acid Genetic Code
  • Mehl, Ryan A.; Anderson, J. Christopher; Santoro, Stephen W.
  • Journal of the American Chemical Society, Vol. 125, Issue 4, p. 935-939 https://doi.org/10.1021/ja0284153
journal January 2003
An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli journal December 2003
An Expanded Eukaryotic Genetic Code journal August 2003
Adaptation of an Orthogonal Archaeal Leucyl-tRNA and Synthetase Pair for Four-base, Amber, and Opal Suppression † journal August 2003
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal August 2002
Introduction of unnatural amino acids into chalcone isomerase journal July 1991
Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli journal March 2001
An efficient system for the evolution of aminoacyl-tRNA synthetase specificity journal September 2002
Functional Annotation of Class I Lysyl-tRNA Synthetase Phylogeny Indicates a Limited Role for Gene Transfer journal August 2002
Complete Sequence and Gene Organization of the Genome of a Hyper-thermophilic Archaebacterium, Pyrococcus horikoshii OT3 journal January 1998
Incorporation of non-natural amino acids into proteins journal December 2002
Introduction of specialty functions by the position-specific incorporation of nonnatural amino acids into proteins through four-base codon/anticodon pairs journal October 2001
A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins journal May 2000
Incorporation of two nonnatural amino acids into proteins through extension of the genetic code journal November 1999
Functional convergence of two lysyl-tRNA synthetases with unrelated topologies journal March 2002
An expanding genetic code journal December 2004
Adding new meanings to the genetic code journal June 2001
Five-base codons for incorporation of nonnatural amino acids into proteins journal September 2001
Evolutionary relationship between Halobacterium cutirubrum and eukaryotes determined by use of aminoacyl-tRNA synthetases as phylogenetic probes journal March 1980
Addition of the keto functional group to the genetic code of Escherichia coli journal December 2002
Expanding the Genetic Code of Escherichia coli journal April 2001
Reading frame selection and transfer RNA anticodon loop stacking journal December 1987
Novel transfer RNAs that are active in Escherichia coli journal May 1992
Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli journal February 1998

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