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Title: Rapid in situ detection of chromosome 21 by PRINS technique

Abstract

The {open_quotes}PRimed IN Situ labeling{close_quotes} (PRINS) method is an interesting alternative to in situ hybridization for chromosomal detection. In this procedure, chromosome labeling is performed by in situ annealing of specific oligonucleotide primers, followed by primer elongation by a Taq polymerase in the presence of labeled nucleotides. Using this process, we have developed a simple and semi-automatic method for rapid in situ detection of human chromosome 21. The reaction was performed on a programmable temperature cycler, with a chromosome 21 specific oligonucleotide primer. Different samples of normal and trisomic lymphocytes and amniotic fluid cells were used for testing the method. Specific labeling of chromosome 21 was obtained in both metaphases and interphase nuclei in a 1 hour reaction. The use of oligonucleotide primer for in situ labeling overcomes the need for complex preparations of specific DNA probes. The present results demonstrate that PRINS may be a simple and reliable technique for rapidly detecting aneuploidies. 18 refs., 1 fig.

Authors:
; ;  [1]
  1. CNRS UPR 9008, Montpellier (France) [and others
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
102882
Resource Type:
Journal Article
Journal Name:
American Journal of Medical Genetics
Additional Journal Information:
Journal Volume: 56; Journal Issue: 4; Other Information: PBD: 8 May 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN CHROMOSOME 21; CHROMOSOMAL ABERRATIONS; ANEUPLOIDY; DETECTION; DNA HYBRIDIZATION; COMPARATIVE EVALUATIONS; AUTOMATION; RELIABILITY; PROBES; FLUORESCENCE; POLYMERASES; OLIGONUCLEOTIDES

Citation Formats

Pellestor, F., Girardet, A., and Andreo, B. Rapid in situ detection of chromosome 21 by PRINS technique. United States: N. p., 1995. Web. doi:10.1002/ajmg.1320560409.
Pellestor, F., Girardet, A., & Andreo, B. Rapid in situ detection of chromosome 21 by PRINS technique. United States. doi:10.1002/ajmg.1320560409.
Pellestor, F., Girardet, A., and Andreo, B. Mon . "Rapid in situ detection of chromosome 21 by PRINS technique". United States. doi:10.1002/ajmg.1320560409.
@article{osti_102882,
title = {Rapid in situ detection of chromosome 21 by PRINS technique},
author = {Pellestor, F. and Girardet, A. and Andreo, B.},
abstractNote = {The {open_quotes}PRimed IN Situ labeling{close_quotes} (PRINS) method is an interesting alternative to in situ hybridization for chromosomal detection. In this procedure, chromosome labeling is performed by in situ annealing of specific oligonucleotide primers, followed by primer elongation by a Taq polymerase in the presence of labeled nucleotides. Using this process, we have developed a simple and semi-automatic method for rapid in situ detection of human chromosome 21. The reaction was performed on a programmable temperature cycler, with a chromosome 21 specific oligonucleotide primer. Different samples of normal and trisomic lymphocytes and amniotic fluid cells were used for testing the method. Specific labeling of chromosome 21 was obtained in both metaphases and interphase nuclei in a 1 hour reaction. The use of oligonucleotide primer for in situ labeling overcomes the need for complex preparations of specific DNA probes. The present results demonstrate that PRINS may be a simple and reliable technique for rapidly detecting aneuploidies. 18 refs., 1 fig.},
doi = {10.1002/ajmg.1320560409},
journal = {American Journal of Medical Genetics},
number = 4,
volume = 56,
place = {United States},
year = {1995},
month = {5}
}