Nanometrology of delignified Populus using mode synthesizing atomic force microscopy

Tetard, Laurene; Passian, Ali; Farahi, R H; Davison, Brian H; Jung, S; Ragauskas, A J; Lereu, Aude; Thundat, Thomas George

doi:10.1088/0957-4484/22/46/465702

Title: Nanometrology of delignified Populus using mode synthesizing atomic force microscopy

Full Record
Other Related Research

Abstract

The study of the spatially resolved physical and compositional properties of materials at the nanoscale is increasingly challenging due to the level of complexity of biological specimens such as those of interest in bioenergy production. Mode synthesizing atomic force microscopy (MSAFM) has emerged as a promising metrology tool for such studies. It is shown that, by tuning the mechanical excitation of the probe-sample system, MSAFM can be used to dynamically investigate the multifaceted complexity of plant cells. The results are argued to be of importance both for the characteristics of the invoked synthesized modes and for accessing new features of the samples. As a specific system to investigate, we present images of Populus, before and after a holopulping treatment, a crucial step in the biomass delignification process.

Authors:

Tetard, Laurene ^[1]; Passian, Ali ^[1]; Farahi, R H ^[1]; Davison, Brian H ^[1]; Jung, S ^[2]; Ragauskas, A J ^[2]; Lereu, Aude ^[3]; Thundat, Thomas George ^[1]

ORNL
Georgia Institute of Technology
CINaM - Centre Interdisciplinaire de Nanoscience de Marseille, France

Publication Date:: Sat Jan 01 00:00:00 EST 2011

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1028756

DOE Contract Number:: DE-AC05-00OR22725

Resource Type:: Journal Article

Journal Name:: Nanotechnology

Additional Journal Information:: Journal Volume: 22; Journal Issue: 46; Journal ID: ISSN 0957-4484

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; ATOMIC FORCE MICROSCOPY; BIOMASS; DELIGNIFICATION; EXCITATION; PLANT CELLS; PRODUCTION; TUNING

Citation Formats


                    Tetard, Laurene, Passian, Ali, Farahi, R H, Davison, Brian H, Jung, S, Ragauskas, A J, Lereu, Aude, and Thundat, Thomas George. Nanometrology of delignified Populus using mode synthesizing atomic force microscopy.  United States: N. p., 2011. 
        Web.  doi:10.1088/0957-4484/22/46/465702.

Copy to clipboard


                    Tetard, Laurene, Passian, Ali, Farahi, R H, Davison, Brian H, Jung, S, Ragauskas, A J, Lereu, Aude, & Thundat, Thomas George. Nanometrology of delignified Populus using mode synthesizing atomic force microscopy.  United States.  https://doi.org/10.1088/0957-4484/22/46/465702

Copy to clipboard


                    Tetard, Laurene, Passian, Ali, Farahi, R H, Davison, Brian H, Jung, S, Ragauskas, A J, Lereu, Aude, and Thundat, Thomas George. 2011.  
        "Nanometrology of delignified Populus using mode synthesizing atomic force microscopy".  United States.  https://doi.org/10.1088/0957-4484/22/46/465702.

Copy to clipboard


                    
@article{osti_1028756,

  title        = {Nanometrology of delignified Populus using mode synthesizing atomic force microscopy},

  author       = {Tetard, Laurene and Passian, Ali and Farahi, R H and Davison, Brian H and Jung, S and Ragauskas, A J and Lereu, Aude and Thundat, Thomas George},

  abstractNote = {The study of the spatially resolved physical and compositional properties of materials at the nanoscale is increasingly challenging due to the level of complexity of biological specimens such as those of interest in bioenergy production. Mode synthesizing atomic force microscopy (MSAFM) has emerged as a promising metrology tool for such studies. It is shown that, by tuning the mechanical excitation of the probe-sample system, MSAFM can be used to dynamically investigate the multifaceted complexity of plant cells. The results are argued to be of importance both for the characteristics of the invoked synthesized modes and for accessing new features of the samples. As a specific system to investigate, we present images of Populus, before and after a holopulping treatment, a crucial step in the biomass delignification process.},

  doi          = {10.1088/0957-4484/22/46/465702},

  url          = {https://www.osti.gov/biblio/1028756},
  journal      = {Nanotechnology},

  issn         = {0957-4484},

  number       = 46,

  volume       = 22,

  place        = {United States},

  year         = {Sat Jan 01 00:00:00 EST 2011},

  month        = {Sat Jan 01 00:00:00 EST 2011}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1088/0957-4484/22/46/465702

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Opto-nanomechanical spectroscopic material characterization

Journal Article Tetard, Laurene; Passian, Ali; Farahi, R.; ... - Nature Nanotechnology

Cellulosic ethanol is a biofuel of considerable potential in the search for sustainable and renewable bioenergy [1,2]. However, while rich in carbohydrates [3], the plant cell walls exhibit a natural resistance to complex phenotype treatments such as enzymatic microbial deconstruction, heat and acid treatments that can remove the lignin polymers from cellulose before hydrolysis [5]. Noninvasive physical and chemical characterization of the cell walls and the effect of such treatments on biomass are challenging but necessary to understand and overcome such resistance [6]. Although lacking chemical recognition in their traditional forms, the various emerging modalities of nano-mechanical [7] and opto-nano-mechanicalmore »« less
Cited by 26
https://doi.org/10.1038/nnano.2015.168

Full Text Available
Plasticity, elasticity, and adhesion energy of plant cell walls: nanometrology of lignin loss using atomic force microscopy

Journal Article Farahi, R.; Charrier, Anne; Tolbert, Allison; ... - Scientific Reports

The complex organic polymer, lignin, abundant in plants, prevents the efficient extraction of sugars from the cell walls that is required for large scale biofuel production. Because lignin removal is crucial in overcoming this challenge, the question of how the nanoscale properties of the plant cell ultrastructure correlate with delignification processes is important. Here, we report how distinct molecular domains can be identified and how physical quantities of adhesion energy, elasticity, and plasticity undergo changes, and whether such quantitative observations can be used to characterize delignification. By chemically processing biomass, and employing nanometrology, the various stages of lignin removal aremore »« less
Cited by 18
https://doi.org/10.1038/s41598-017-00234-4

Full Text Available
Nanometrology of Biomass for Bioenergy: The Role of Atomic Force Microscopy and Spectroscopy in Plant Cell Characterization

Journal Article Charrier, Anne; Lereu, Aude; Farahi, Rubye; ... - Frontiers in Energy Research

Ethanol production using extracted cellulose from plant cell walls (PCW) is a very promising approach to biofuel production. However, efficient throughput has been hindered by the phenomenon of recalcitrance, leading to high costs for the lignocellulosic conversion. To overcome recalcitrance, it is necessary to understand the chemical and structural properties of the plant biological materials, which have evolved to generate the strong and cohesive features observed in plants. Therefore, tools and methods that allow the investigation of how the different molecular components of PCW are organized and distributed and how this impacts the mechanical properties of the plants are neededmore »« less
Cited by 11
https://doi.org/10.3389/fenrg.2018.00011

Full Text Available
Advances in quantitative nanoscale subsurface imaging by mode-synthesizing atomic force microscopy

Journal Article Vitry, P.; Bourillot, E.; Plassard, C.; ... - Applied Physics Letters

This paper reports on advances toward quantitative non-destructive nanoscale subsurface investigation of a nanofabricated sample based on mode synthesizing atomic force microscopy with heterodyne detection, addressing the need to correlate the role of actuation frequencies of the probe f{sub p} and the sample f{sub s} with depth resolution for 3D tomography reconstruction. Here, by developing a simple model and validating the approach experimentally through the study of the nanofabricated calibration depth samples consisting of buried metallic patterns, we demonstrate avenues for quantitative nanoscale subsurface imaging. Our findings enable the reconstruction of the sample depth profile and allow high fidelity resolutionmore »« less
https://doi.org/10.1063/1.4892467
Applications of Subsurface Microscopy

Book Tetard, Laurene; Passian, Ali; Farahi, R; ...

Exploring the interior of a cell is of tremendous importance in order to assess the effects of nanomaterials on biological systems. Outside of a controlled laboratory environment, nanomaterials will most likely not be conveniently labeled or tagged so that their translocation within a biological system cannot be easily identified and quantified. Ideally, the characterization of nanomaterials within a cell requires a nondestructive, label-free, and subsurface approach. Subsurface nanoscale imaging represents a real challenge for instrumentation. Indeed the tools available for high resolution characterization, including optical, electron or scanning probe microscopies, mainly provide topography images or require tagants that fluoresce. Althoughmore »« less

Similar Records