skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Capillary electrophoresis systems and methods

Abstract

An embodiment of the invention is directed to a capillary electrophoresis apparatus comprising a plurality of separation micro-channels. A sample loading channel communicates with each of the plurality of separation channels. A driver circuit comprising a plurality of electrodes is configured to induce an electric field across each of the plurality of separation channels sufficient to cause analytes in the samples to migrate along each of the channels. The system further comprises a plurality of detectors configured to detect the analytes.

Inventors:
 [1];  [2];  [2];  [3];  [4];  [2];  [5];  [2];  [6]
  1. Hillsboro, OR
  2. Louisville, KY
  3. Georgetown, IN
  4. New Albany, IN
  5. Goshen, KY
  6. Mount Washington, KY
Publication Date:
Research Org.:
University of Louisville Research Foundation, Inc. (Louisville, KY)
Sponsoring Org.:
USDOE
OSTI Identifier:
1026843
Patent Number(s):
7,988,839
Application Number:
11/524,357
Assignee:
University of Louisville Research Foundation, Inc. (Louisville, KY) OSTI
DOE Contract Number:
46411101095
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Dorairaj, Rathissh, Keynton, Robert S, Roussel, Thomas J, Crain, Mark M, Jackson, Douglas J, Walsh, Kevin M, Naber, John F, Baldwin, Richard P, and Franco, Danielle B. Capillary electrophoresis systems and methods. United States: N. p., 2011. Web.
Dorairaj, Rathissh, Keynton, Robert S, Roussel, Thomas J, Crain, Mark M, Jackson, Douglas J, Walsh, Kevin M, Naber, John F, Baldwin, Richard P, & Franco, Danielle B. Capillary electrophoresis systems and methods. United States.
Dorairaj, Rathissh, Keynton, Robert S, Roussel, Thomas J, Crain, Mark M, Jackson, Douglas J, Walsh, Kevin M, Naber, John F, Baldwin, Richard P, and Franco, Danielle B. Tue . "Capillary electrophoresis systems and methods". United States. doi:. https://www.osti.gov/servlets/purl/1026843.
@article{osti_1026843,
title = {Capillary electrophoresis systems and methods},
author = {Dorairaj, Rathissh and Keynton, Robert S and Roussel, Thomas J and Crain, Mark M and Jackson, Douglas J and Walsh, Kevin M and Naber, John F and Baldwin, Richard P and Franco, Danielle B},
abstractNote = {An embodiment of the invention is directed to a capillary electrophoresis apparatus comprising a plurality of separation micro-channels. A sample loading channel communicates with each of the plurality of separation channels. A driver circuit comprising a plurality of electrodes is configured to induce an electric field across each of the plurality of separation channels sufficient to cause analytes in the samples to migrate along each of the channels. The system further comprises a plurality of detectors configured to detect the analytes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 02 00:00:00 EDT 2011},
month = {Tue Aug 02 00:00:00 EDT 2011}
}

Patent:

Save / Share:
  • The present invention is an interface between a capillary electrophoresis separation capillary end and an electrospray ionization mass spectrometry emitter capillary end, for transporting an analyte sample from a capillary electrophoresis separation capillary to a electrospray ionization mass spectrometry emitter capillary. The interface of the present invention has: (a) a charge transfer fitting enclosing both of the capillary electrophoresis capillary end and the electrospray ionization mass spectrometry emitter capillary end; (b) a reservoir containing an electrolyte surrounding the charge transfer fitting; and (c) an electrode immersed into the electrolyte, the electrode closing a capillary electrophoresis circuit and providing charge transfermore » across the charge transfer fitting while avoiding substantial bulk fluid transfer across the charge transfer fitting. Advantages of the present invention have been demonstrated as effective in providing high sensitivity and efficient analyses.« less
  • A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. This multiplexing approach involves laser irradiation of a sample in a plurality of capillaries through optical fibers that are coupled individually with the capillaries. The array is imaged orthogonally through a microscope onto a charge-coupled device camera for signal analysis. 14 figs.
  • A device for providing equal electrical potential between two loci unconnected by solid or liquid electrical conductors is provided. The device comprises a first electrical conducting terminal, a second electrical conducting terminal connected to the first terminal by a rigid dielectric structure, and an electrically conducting gas contacting the first and second terminals. This device is particularly suitable for application in the electrospray ionization interface between a capillary zone electrophoresis apparatus and a mass spectrometer. 1 fig.
  • Capillary electrophoresis (CE) is interfaced with low temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization of separated molecular analytes. 21 figs.
  • The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A charge-injection device is optically coupled to capillaries in the array such that the interior of a capillary is imaged onto only one pixel. In Sanger-type 4-label DNA sequencing reactions, nucleotide identification (``base calling``) is improved by using two long-pass filters to split fluorescence emission into two emission channels. A binary poly(ethyleneoxide) matrix is used in the electrophoretic separations. 19 figs.