skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: 'Effects of Elevated Temperature on Dehalococcoides Dechlorination Performance and DNA and RNA Biomarker Abundance

Abstract

Coupling thermal treatment with microbial reductive dechlorination is a promising remedy for tetrachloroethene (PCE) and trichloroethene (TCE) contaminated source zones. Laboratory experiments evaluated Dehalococcoides (Dhc) dechlorination performance, viability, and biomarker gene (DNA) and transcript (mRNA) abundances during exposure to elevated temperatures. The PCE-dechlorinating consortia BDI and OW produced ethene when incubated at temperatures of 30 C, but vinyl chloride (VC) accumulated when cultures were incubated at 35 or 40 C. Cultures incubated at 40 C for less than 49 days resumed VC dechlorination following cooling; however, incubation at 45 C resulted in complete loss of dechlorination activity. Dhc 16S rRNA, bvcA, and vcrA gene abundances in cultures showing complete dechlorination to ethene at 30 C exceeded those measured in cultures incubated at higher temperatures, consistent with observed dechlorination activities. Conversely, biomarker gene transcript abundances per cell in cultures incubated at 35 and 40 C were generally at least one order-of-magnitude greater than those measured in ethene-producing cultures incubated at 30 C. Even in cultures accumulating VC, transcription of the vcrA gene, which is implicated in VC-to-ethene dechlorination, was up-regulated. Temperature stress caused the up-regulation of Dhc reductive dehalogenase gene expression indicating that Dhc gene expression measurements should be interpreted cautiouslymore » as Dhc biomarker gene transcript abundances may not correlate with dechlorination activity.« less

Authors:
 [1];  [2];  [2];  [2];  [2];  [1]
  1. ORNL
  2. University of Tennessee
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1025877
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Environmental Science & Technology
Additional Journal Information:
Journal Volume: 001; Journal Issue: 001; Journal ID: ISSN 0013-936X
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 54 ENVIRONMENTAL SCIENCES; ABUNDANCE; BIOLOGICAL MARKERS; BIOREMEDIATION; CHLORINATED ALIPHATIC HYDROCARBONS; DECHLORINATION; DNA; GENES; HEAT TREATMENTS; INCUBATION; PERFORMANCE; RNA; TEMPERATURE RANGE 0273-0400 K; TRANSCRIPTION; VIABILITY; VINYL CHLORIDE

Citation Formats

Fletcher, Kelly E, Costanza, Jed, Cruz-Garcia, Claribel, Ramaswamy, Nivedhya, Pennell, Kurt, and Loeffler, Frank E. 'Effects of Elevated Temperature on Dehalococcoides Dechlorination Performance and DNA and RNA Biomarker Abundance. United States: N. p., 2011. Web.
Fletcher, Kelly E, Costanza, Jed, Cruz-Garcia, Claribel, Ramaswamy, Nivedhya, Pennell, Kurt, & Loeffler, Frank E. 'Effects of Elevated Temperature on Dehalococcoides Dechlorination Performance and DNA and RNA Biomarker Abundance. United States.
Fletcher, Kelly E, Costanza, Jed, Cruz-Garcia, Claribel, Ramaswamy, Nivedhya, Pennell, Kurt, and Loeffler, Frank E. 2011. "'Effects of Elevated Temperature on Dehalococcoides Dechlorination Performance and DNA and RNA Biomarker Abundance". United States.
@article{osti_1025877,
title = {'Effects of Elevated Temperature on Dehalococcoides Dechlorination Performance and DNA and RNA Biomarker Abundance},
author = {Fletcher, Kelly E and Costanza, Jed and Cruz-Garcia, Claribel and Ramaswamy, Nivedhya and Pennell, Kurt and Loeffler, Frank E},
abstractNote = {Coupling thermal treatment with microbial reductive dechlorination is a promising remedy for tetrachloroethene (PCE) and trichloroethene (TCE) contaminated source zones. Laboratory experiments evaluated Dehalococcoides (Dhc) dechlorination performance, viability, and biomarker gene (DNA) and transcript (mRNA) abundances during exposure to elevated temperatures. The PCE-dechlorinating consortia BDI and OW produced ethene when incubated at temperatures of 30 C, but vinyl chloride (VC) accumulated when cultures were incubated at 35 or 40 C. Cultures incubated at 40 C for less than 49 days resumed VC dechlorination following cooling; however, incubation at 45 C resulted in complete loss of dechlorination activity. Dhc 16S rRNA, bvcA, and vcrA gene abundances in cultures showing complete dechlorination to ethene at 30 C exceeded those measured in cultures incubated at higher temperatures, consistent with observed dechlorination activities. Conversely, biomarker gene transcript abundances per cell in cultures incubated at 35 and 40 C were generally at least one order-of-magnitude greater than those measured in ethene-producing cultures incubated at 30 C. Even in cultures accumulating VC, transcription of the vcrA gene, which is implicated in VC-to-ethene dechlorination, was up-regulated. Temperature stress caused the up-regulation of Dhc reductive dehalogenase gene expression indicating that Dhc gene expression measurements should be interpreted cautiously as Dhc biomarker gene transcript abundances may not correlate with dechlorination activity.},
doi = {},
url = {https://www.osti.gov/biblio/1025877}, journal = {Environmental Science & Technology},
issn = {0013-936X},
number = 001,
volume = 001,
place = {United States},
year = {2011},
month = {1}
}