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Title: Phloem transport of arsenic species from flag leaf to grain during grain filling

Abstract

Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was investigated. Arsenic species were delivered through cut flag leaves during grain fill. Spatial unloading within grains was investigated using synchrotron X-ray fluorescence (SXRF) microtomography. Additionally, the effect of germanic acid (a silicic acid analog) on grain As accumulation in arsenite-treated panicles was examined. Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were extremely efficiently retranslocated from flag leaves to rice grain; arsenate was poorly retranslocated, and was rapidly reduced to arsenite within flag leaves; arsenite displayed no retranslocation. Within grains, DMA rapidly dispersed while MMA and inorganic As remained close to the entry point. Germanic acid addition did not affect grain As in arsenite-treated panicles. Three-dimensional SXRF microtomography gave further information on arsenite localization in the ovular vascular trace (OVT) of rice grains. These results demonstrate that inorganic As is poorly remobilized, while organic species are readily remobilized, from leaves to grain. Stem translocation of inorganic As may not rely solely on silicic acid transporters.

Authors:
; ; ; ; ; ; ; ; ; ; ;  [1];  [2];  [2];  [2];  [2]
  1. (EPA)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
EPANIH
OSTI Identifier:
1024500
Resource Type:
Journal Article
Journal Name:
New Phytol.
Additional Journal Information:
Journal Volume: 192; Journal Issue: (1) ; 10, 2011; Journal ID: ISSN 0028-646X
Country of Publication:
United States
Language:
ENGLISH
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; ARSENATES; ARSENIC; FLUORESCENCE; RICE; SILICIC ACID; SYNCHROTRONS; TRANSLOCATION; TRANSPORT; UNLOADING

Citation Formats

Carey, Anne-Marie, Norton, Gareth J., Deacon, Claire, Scheckel, Kirk G., Lombi, Enzo, Punshon, Tracy, Guerinot, Mary Lou, Lanzirotti, Antonio, Newville, Matt, Choi, Yongseong, Price, Adam H., Meharg, Andrew A., U. South Australia), Aberdeen), UC), and Dartmouth). Phloem transport of arsenic species from flag leaf to grain during grain filling. United States: N. p., 2011. Web. doi:10.1111/j.1469-8137.2011.03789.x.
Carey, Anne-Marie, Norton, Gareth J., Deacon, Claire, Scheckel, Kirk G., Lombi, Enzo, Punshon, Tracy, Guerinot, Mary Lou, Lanzirotti, Antonio, Newville, Matt, Choi, Yongseong, Price, Adam H., Meharg, Andrew A., U. South Australia), Aberdeen), UC), & Dartmouth). Phloem transport of arsenic species from flag leaf to grain during grain filling. United States. doi:10.1111/j.1469-8137.2011.03789.x.
Carey, Anne-Marie, Norton, Gareth J., Deacon, Claire, Scheckel, Kirk G., Lombi, Enzo, Punshon, Tracy, Guerinot, Mary Lou, Lanzirotti, Antonio, Newville, Matt, Choi, Yongseong, Price, Adam H., Meharg, Andrew A., U. South Australia), Aberdeen), UC), and Dartmouth). Tue . "Phloem transport of arsenic species from flag leaf to grain during grain filling". United States. doi:10.1111/j.1469-8137.2011.03789.x.
@article{osti_1024500,
title = {Phloem transport of arsenic species from flag leaf to grain during grain filling},
author = {Carey, Anne-Marie and Norton, Gareth J. and Deacon, Claire and Scheckel, Kirk G. and Lombi, Enzo and Punshon, Tracy and Guerinot, Mary Lou and Lanzirotti, Antonio and Newville, Matt and Choi, Yongseong and Price, Adam H. and Meharg, Andrew A. and U. South Australia) and Aberdeen) and UC) and Dartmouth)},
abstractNote = {Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was investigated. Arsenic species were delivered through cut flag leaves during grain fill. Spatial unloading within grains was investigated using synchrotron X-ray fluorescence (SXRF) microtomography. Additionally, the effect of germanic acid (a silicic acid analog) on grain As accumulation in arsenite-treated panicles was examined. Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were extremely efficiently retranslocated from flag leaves to rice grain; arsenate was poorly retranslocated, and was rapidly reduced to arsenite within flag leaves; arsenite displayed no retranslocation. Within grains, DMA rapidly dispersed while MMA and inorganic As remained close to the entry point. Germanic acid addition did not affect grain As in arsenite-treated panicles. Three-dimensional SXRF microtomography gave further information on arsenite localization in the ovular vascular trace (OVT) of rice grains. These results demonstrate that inorganic As is poorly remobilized, while organic species are readily remobilized, from leaves to grain. Stem translocation of inorganic As may not rely solely on silicic acid transporters.},
doi = {10.1111/j.1469-8137.2011.03789.x},
journal = {New Phytol.},
issn = {0028-646X},
number = (1) ; 10, 2011,
volume = 192,
place = {United States},
year = {2011},
month = {9}
}