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Title: Proteome Studies of Filamentous Fungi

Abstract

The continued fast pace of fungal genome sequence generation has enabled proteomic analysis of a wide breadth of organisms that span the breadth of the Kingdom Fungi. There is some phylogenetic bias to the current catalog of fungi with reasonable DNA sequence databases (genomic or EST) that could be analyzed at a global proteomic level. However, the rapid development of next generation sequencing platforms has lowered the cost of genome sequencing such that in the near future, having a genome sequence will no longer be a time or cost bottleneck for downstream proteomic (and transcriptomic) analyses. High throughput, non-gel based proteomics offers a snapshot of proteins present in a given sample at a single point in time. There are a number of different variations on the general method and technologies for identifying peptides in a given sample. We present a method that can serve as a “baseline” for proteomic studies of fungi.

Authors:
;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1023725
Report Number(s):
PNNL-SA-78038
BM0101020; TRN: US201120%%1061
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Book
Resource Relation:
Related Information: Fungal Genomics: Methods and Protocols. Methods in Molecular Biology, 722:133-139
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; COST; DNA; FUNGI; PEPTIDES; PROTEINS; VARIATIONS; proteomics; fungi; gene models

Citation Formats

Baker, Scott E., and Panisko, Ellen A. Proteome Studies of Filamentous Fungi. United States: N. p., 2011. Web.
Baker, Scott E., & Panisko, Ellen A. Proteome Studies of Filamentous Fungi. United States.
Baker, Scott E., and Panisko, Ellen A. Wed . "Proteome Studies of Filamentous Fungi". United States.
@article{osti_1023725,
title = {Proteome Studies of Filamentous Fungi},
author = {Baker, Scott E. and Panisko, Ellen A.},
abstractNote = {The continued fast pace of fungal genome sequence generation has enabled proteomic analysis of a wide breadth of organisms that span the breadth of the Kingdom Fungi. There is some phylogenetic bias to the current catalog of fungi with reasonable DNA sequence databases (genomic or EST) that could be analyzed at a global proteomic level. However, the rapid development of next generation sequencing platforms has lowered the cost of genome sequencing such that in the near future, having a genome sequence will no longer be a time or cost bottleneck for downstream proteomic (and transcriptomic) analyses. High throughput, non-gel based proteomics offers a snapshot of proteins present in a given sample at a single point in time. There are a number of different variations on the general method and technologies for identifying peptides in a given sample. We present a method that can serve as a “baseline” for proteomic studies of fungi.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {4}
}

Book:
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