Thioredoxin-dependent Redox Regulation of Cellular Signaling and Stress Response through Reversible Oxidation of Methionines
Abstract
Generation of reactive oxygen species (ROS) is a common feature of many forms of stress to which plants are exposed. Successful adaptation to changing environmental conditions requires sensitive sensors of ROS such as protein-bound methionines that are converted to their corresponding methionine sulfoxides, which in turn can influence cellular signaling pathways. Such a signaling protein is calmodulin, which represents an early and central point in calcium signaling pathways important to stress response in plants. We describe recent work elucidating fundamental mechanisms of reversible methionine oxidation within calmodulin, including the sensitivity of individual methionines within plant and animal calmodulin to ROS, the structural and functional consequences of their oxidation, and the interactions of oxidized calmodulin with methionine sulfoxide reductase enzymes.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1023721
- Report Number(s):
- PNNL-SA-75372
400403909; TRN: US201120%%1057
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Molecular Biosystems, 7(7):2101-2109
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 7
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ANIMALS; CALCIUM; CALMODULIN; ENZYMES; FUNCTIONALS; INTERACTIONS; METHIONINE; OXIDATION; OXIDOREDUCTASES; OXYGEN; PLANTS; PROTEINS; SENSITIVITY; SENSORS; SULFOXIDES
Citation Formats
Bigelow, Diana J, and Squier, Thomas C. Thioredoxin-dependent Redox Regulation of Cellular Signaling and Stress Response through Reversible Oxidation of Methionines. United States: N. p., 2011.
Web. doi:10.1039/C1MB05081H.
Bigelow, Diana J, & Squier, Thomas C. Thioredoxin-dependent Redox Regulation of Cellular Signaling and Stress Response through Reversible Oxidation of Methionines. United States. https://doi.org/10.1039/C1MB05081H
Bigelow, Diana J, and Squier, Thomas C. 2011.
"Thioredoxin-dependent Redox Regulation of Cellular Signaling and Stress Response through Reversible Oxidation of Methionines". United States. https://doi.org/10.1039/C1MB05081H.
@article{osti_1023721,
title = {Thioredoxin-dependent Redox Regulation of Cellular Signaling and Stress Response through Reversible Oxidation of Methionines},
author = {Bigelow, Diana J and Squier, Thomas C},
abstractNote = {Generation of reactive oxygen species (ROS) is a common feature of many forms of stress to which plants are exposed. Successful adaptation to changing environmental conditions requires sensitive sensors of ROS such as protein-bound methionines that are converted to their corresponding methionine sulfoxides, which in turn can influence cellular signaling pathways. Such a signaling protein is calmodulin, which represents an early and central point in calcium signaling pathways important to stress response in plants. We describe recent work elucidating fundamental mechanisms of reversible methionine oxidation within calmodulin, including the sensitivity of individual methionines within plant and animal calmodulin to ROS, the structural and functional consequences of their oxidation, and the interactions of oxidized calmodulin with methionine sulfoxide reductase enzymes.},
doi = {10.1039/C1MB05081H},
url = {https://www.osti.gov/biblio/1023721},
journal = {Molecular Biosystems, 7(7):2101-2109},
number = 7,
volume = 7,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 2011},
month = {Wed Jun 01 00:00:00 EDT 2011}
}