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Title: High-Resolution Mosaic Imaging with Multifocal, Multiphoton Photon-Counting Microscopy

Abstract

High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO{sub 4} laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1023696
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Journal Name:
Applied Optics
Additional Journal Information:
Journal Volume: 48; Journal Issue: 11, 10 April 2009; Journal ID: ISSN 0003-6935
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; COUNTING RATES; DESIGN; IMAGE PROCESSING; LASERS; MICROSCOPES; OPTICAL MICROSCOPY; MODE LOCKING; MULTI-PHOTON PROCESSES; MULTIPLEXERS; PHOTONS; YTTERBIUM; Chemical and Biosciences

Citation Formats

Chandler, E, Hoover, E, Field, J, Sheetz, K, Amir, W, Carriles, R, Ding, S Y, and Squier, J. High-Resolution Mosaic Imaging with Multifocal, Multiphoton Photon-Counting Microscopy. United States: N. p., 2009. Web. doi:10.1364/AO.48.002067.
Chandler, E, Hoover, E, Field, J, Sheetz, K, Amir, W, Carriles, R, Ding, S Y, & Squier, J. High-Resolution Mosaic Imaging with Multifocal, Multiphoton Photon-Counting Microscopy. United States. https://doi.org/10.1364/AO.48.002067
Chandler, E, Hoover, E, Field, J, Sheetz, K, Amir, W, Carriles, R, Ding, S Y, and Squier, J. 2009. "High-Resolution Mosaic Imaging with Multifocal, Multiphoton Photon-Counting Microscopy". United States. https://doi.org/10.1364/AO.48.002067.
@article{osti_1023696,
title = {High-Resolution Mosaic Imaging with Multifocal, Multiphoton Photon-Counting Microscopy},
author = {Chandler, E and Hoover, E and Field, J and Sheetz, K and Amir, W and Carriles, R and Ding, S Y and Squier, J},
abstractNote = {High-resolution mosaic imaging is performed for the first time to our knowledge with a multifocal, multiphoton, photon-counting imaging system. We present a novel design consisting of a home-built femtosecond Yb-doped KGdWO{sub 4} laser with an optical multiplexer, which is coupled with a commercial Olympus IX-71 microscope frame. Photon counting is performed using single-element detectors and an inexpensive electronic demultiplexer and counters.},
doi = {10.1364/AO.48.002067},
url = {https://www.osti.gov/biblio/1023696}, journal = {Applied Optics},
issn = {0003-6935},
number = 11, 10 April 2009,
volume = 48,
place = {United States},
year = {Fri Apr 10 00:00:00 EDT 2009},
month = {Fri Apr 10 00:00:00 EDT 2009}
}