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Title: Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis

Abstract

Glycosyltransferase family14 (GT14) belongs to the glycosyltransferase (GT) superfamily that plays important roles in the biosynthesis of cell walls, the most abundant source of cellulosic biomass for bioethanol production. It has been hypothesized that DUF266 proteins are a new class of GTs related to GT14. In this study, we identified 62 GT14 and 106 DUF266 genes (named GT14-like herein) in Arabidopsis, Oryza, Populus, Sorghum and Vitis. Our phylogenetic analysis separated GT14 and GT14-like genes into two distinct clades, which were further divided into eight and five groups, respectively. Similarities in protein domain, 3D structure and gene expression were uncovered between the two phylogenetic clades, supporting the hypothesis that GT14 and GT14-like genes belong to one family. Therefore, we proposed a new family name, GT14/GT14-like family that combines both subfamilies. Variation in gene expression and protein subcellular localization within the GT14-like subfamily were greater than those within the GT14 subfamily. One-half of the Arabidopsis and Populus GT14/GT14-like genes were found to be preferentially expressed in stem/xylem, indicating that they are likely involved in cell wall biosynthesis. This study provided new insights into the evolution and functional diversification of the GT14/GT14-like family genes.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1023294
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Plant Science
Additional Journal Information:
Journal Volume: In press; Journal Issue: 6
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ARABIDOPSIS; BIOETHANOL; BIOMASS; BIOSYNTHESIS; CELL WALL; DIVERSIFICATION; FUNCTIONALS; GENES; HYPOTHESIS; PRODUCTION; PROTEINS; RICE; SORGHUM

Citation Formats

Ye, Chuyu, Li, Ting, Tuskan, Gerald A, Tschaplinski, Timothy J, and Yang, Xiaohan. Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis. United States: N. p., 2011. Web. doi:10.1016/j.plantsci.2011.01.021.
Ye, Chuyu, Li, Ting, Tuskan, Gerald A, Tschaplinski, Timothy J, & Yang, Xiaohan. Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis. United States. https://doi.org/10.1016/j.plantsci.2011.01.021
Ye, Chuyu, Li, Ting, Tuskan, Gerald A, Tschaplinski, Timothy J, and Yang, Xiaohan. 2011. "Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis". United States. https://doi.org/10.1016/j.plantsci.2011.01.021.
@article{osti_1023294,
title = {Comparative analysis of GT14/GT14-like family genes in Arabidopsis, Oryza, Populus, Sorghum and Vitis},
author = {Ye, Chuyu and Li, Ting and Tuskan, Gerald A and Tschaplinski, Timothy J and Yang, Xiaohan},
abstractNote = {Glycosyltransferase family14 (GT14) belongs to the glycosyltransferase (GT) superfamily that plays important roles in the biosynthesis of cell walls, the most abundant source of cellulosic biomass for bioethanol production. It has been hypothesized that DUF266 proteins are a new class of GTs related to GT14. In this study, we identified 62 GT14 and 106 DUF266 genes (named GT14-like herein) in Arabidopsis, Oryza, Populus, Sorghum and Vitis. Our phylogenetic analysis separated GT14 and GT14-like genes into two distinct clades, which were further divided into eight and five groups, respectively. Similarities in protein domain, 3D structure and gene expression were uncovered between the two phylogenetic clades, supporting the hypothesis that GT14 and GT14-like genes belong to one family. Therefore, we proposed a new family name, GT14/GT14-like family that combines both subfamilies. Variation in gene expression and protein subcellular localization within the GT14-like subfamily were greater than those within the GT14 subfamily. One-half of the Arabidopsis and Populus GT14/GT14-like genes were found to be preferentially expressed in stem/xylem, indicating that they are likely involved in cell wall biosynthesis. This study provided new insights into the evolution and functional diversification of the GT14/GT14-like family genes.},
doi = {10.1016/j.plantsci.2011.01.021},
url = {https://www.osti.gov/biblio/1023294}, journal = {Plant Science},
number = 6,
volume = In press,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2011},
month = {Sat Jan 01 00:00:00 EST 2011}
}