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Title: Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics

Abstract

Liquid chromatography-mass spectrometry (LC-MS)-based quantitative proteomics has become increasingly applied for a broad range of biological applications due to growing capabilities for broad proteome coverage and good accuracy in quantification. Herein, we review the current LC-MS-based quantification methods with respect to their advantages and limitations, and highlight their potential applications.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1023115
Report Number(s):
PNNL-SA-76123
Journal ID: ISSN 0021-9258; JBCHA3; 24698; 400412000; TRN: US201118%%763
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of Biological Chemistry, 286(29):25443-25449
Additional Journal Information:
Journal Volume: 286; Journal Issue: 29; Journal ID: ISSN 0021-9258
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; ACCURACY; MASS SPECTROSCOPY; LIQUID COLUMN CHROMATOGRAPHY; PROTEINS; Environmental Molecular Sciences Laboratory

Citation Formats

Xie, Fang, Liu, Tao, Qian, Weijun, Petyuk, Vladislav A., and Smith, Richard D.. Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics. United States: N. p., 2011. Web. doi:10.1074/jbc.R110.199703.
Xie, Fang, Liu, Tao, Qian, Weijun, Petyuk, Vladislav A., & Smith, Richard D.. Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics. United States. doi:10.1074/jbc.R110.199703.
Xie, Fang, Liu, Tao, Qian, Weijun, Petyuk, Vladislav A., and Smith, Richard D.. Fri . "Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics". United States. doi:10.1074/jbc.R110.199703.
@article{osti_1023115,
title = {Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics},
author = {Xie, Fang and Liu, Tao and Qian, Weijun and Petyuk, Vladislav A. and Smith, Richard D.},
abstractNote = {Liquid chromatography-mass spectrometry (LC-MS)-based quantitative proteomics has become increasingly applied for a broad range of biological applications due to growing capabilities for broad proteome coverage and good accuracy in quantification. Herein, we review the current LC-MS-based quantification methods with respect to their advantages and limitations, and highlight their potential applications.},
doi = {10.1074/jbc.R110.199703},
journal = {Journal of Biological Chemistry, 286(29):25443-25449},
issn = {0021-9258},
number = 29,
volume = 286,
place = {United States},
year = {2011},
month = {7}
}