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Title: A nonself sugar mimic of the HIV glycan shield shows enhanced antigenicity

Abstract

Antibody 2G12 uniquely neutralizes a broad range of HIV-1 isolates by binding the high-mannose glycans on the HIV-1 surface glycoprotein, gp120. Antigens that resemble these natural epitopes of 2G12 would be highly desirable components for an HIV-1 vaccine. However, host-produced (self)-carbohydrate motifs have been unsuccessful so far at eliciting 2G12-like antibodies that cross-react with gp120. Based on the surprising observation that 2G12 binds nonproteinaceous monosaccharide D-fructose with higher affinity than D-mannose, we show here that a designed set of nonself, synthetic monosaccharides are potent antigens. When introduced to the terminus of the D1 arm of protein glycans recognized by 2G12, their antigenicity is significantly enhanced. Logical variation of these unnatural sugars pinpointed key modifications, and the molecular basis of this increased antigenicity was elucidated using high-resolution crystallographic analyses. Virus-like particle protein conjugates containing such nonself glycans are bound more tightly by 2G12. As immunogens they elicit higher titers of antibodies than those immunogenic conjugates containing the self D1 glycan motif. These antibodies generated from nonself immunogens also cross-react with this self motif, which is found in the glycan shield, when it is presented in a range of different conjugates and glycans. However, these antibodies did not bind this glycan motifmore » when present on gp120.« less

Authors:
; ; ; ; ; ; ; ; ;  [1]
  1. Scripps
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
OTHERNIH
OSTI Identifier:
1023035
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. USA
Additional Journal Information:
Journal Volume: 107; Journal Issue: (40) ; 10, 2010; Journal ID: ISSN 1091-6490
Country of Publication:
United States
Language:
ENGLISH
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; AFFINITY; AIDS VIRUS; ANTIBODIES; ANTIGENS; MODIFICATIONS; MONOSACCHARIDES; PROTEINS; SACCHARIDES; SACCHAROSE; SHIELDS

Citation Formats

Doores, Katie J, Fulton, Zara, Hong, Vu, Patel, Mitul K, Scanlan, Christopher N, Wormald, Mark R, Finn, M G, Burton, Dennis R, Wilson, Ian A, Davis, Benjamin G, and Oxford). A nonself sugar mimic of the HIV glycan shield shows enhanced antigenicity. United States: N. p., 2011. Web. doi:10.1073/pnas.1002717107.
Doores, Katie J, Fulton, Zara, Hong, Vu, Patel, Mitul K, Scanlan, Christopher N, Wormald, Mark R, Finn, M G, Burton, Dennis R, Wilson, Ian A, Davis, Benjamin G, & Oxford). A nonself sugar mimic of the HIV glycan shield shows enhanced antigenicity. United States. https://doi.org/10.1073/pnas.1002717107
Doores, Katie J, Fulton, Zara, Hong, Vu, Patel, Mitul K, Scanlan, Christopher N, Wormald, Mark R, Finn, M G, Burton, Dennis R, Wilson, Ian A, Davis, Benjamin G, and Oxford). Wed . "A nonself sugar mimic of the HIV glycan shield shows enhanced antigenicity". United States. https://doi.org/10.1073/pnas.1002717107.
@article{osti_1023035,
title = {A nonself sugar mimic of the HIV glycan shield shows enhanced antigenicity},
author = {Doores, Katie J and Fulton, Zara and Hong, Vu and Patel, Mitul K and Scanlan, Christopher N and Wormald, Mark R and Finn, M G and Burton, Dennis R and Wilson, Ian A and Davis, Benjamin G and Oxford)},
abstractNote = {Antibody 2G12 uniquely neutralizes a broad range of HIV-1 isolates by binding the high-mannose glycans on the HIV-1 surface glycoprotein, gp120. Antigens that resemble these natural epitopes of 2G12 would be highly desirable components for an HIV-1 vaccine. However, host-produced (self)-carbohydrate motifs have been unsuccessful so far at eliciting 2G12-like antibodies that cross-react with gp120. Based on the surprising observation that 2G12 binds nonproteinaceous monosaccharide D-fructose with higher affinity than D-mannose, we show here that a designed set of nonself, synthetic monosaccharides are potent antigens. When introduced to the terminus of the D1 arm of protein glycans recognized by 2G12, their antigenicity is significantly enhanced. Logical variation of these unnatural sugars pinpointed key modifications, and the molecular basis of this increased antigenicity was elucidated using high-resolution crystallographic analyses. Virus-like particle protein conjugates containing such nonself glycans are bound more tightly by 2G12. As immunogens they elicit higher titers of antibodies than those immunogenic conjugates containing the self D1 glycan motif. These antibodies generated from nonself immunogens also cross-react with this self motif, which is found in the glycan shield, when it is presented in a range of different conjugates and glycans. However, these antibodies did not bind this glycan motif when present on gp120.},
doi = {10.1073/pnas.1002717107},
url = {https://www.osti.gov/biblio/1023035}, journal = {Proc. Natl. Acad. Sci. USA},
issn = {1091-6490},
number = (40) ; 10, 2010,
volume = 107,
place = {United States},
year = {2011},
month = {8}
}