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Title: X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection

Abstract

The X-CHIP (X-ray Crystallization High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The system has been designed for crystallization condition screening, visual crystal inspection, initial X-ray screening and data collection in a high-throughput fashion. X-ray diffraction data acquisition can be performed directly on-the-chip at room temperature using an in situ approach. The capabilities of the chip eliminate the necessity for manual crystal handling and cryoprotection of crystal samples, while allowing data collection from multiple crystals in the same drop. This technology would be especially beneficial for projects with large volumes of data, such as protein-complex studies and fragment-based screening. The platform employs hydrophilic and hydrophobic concentric ring surfaces on a miniature plate transparent to visible light and X-rays to create a well defined and stable microbatch crystallization environment. The results of crystallization and data-collection experiments demonstrate that high-quality well diffracting crystals can be grown and high-resolution diffraction data sets can be collected using this technology. Furthermore, the quality of a single-wavelength anomalous dispersion data set collected with the X-CHIP at room temperature was sufficientmore » to generate interpretable electron-density maps. This technology is highly resource-efficient owing to the use of nanolitre-scale drop volumes. It does not require any modification for most in-house and synchrotron beamline systems and offers a promising opportunity for full automation of the X-ray structure-determination process.« less

Authors:
; ; ; ; ; ; ;  [1];  [2];  [2]
  1. (Toronto)
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGNINDUSTRY
OSTI Identifier:
1020578
Resource Type:
Journal Article
Journal Name:
Acta Crystallogr. D
Additional Journal Information:
Journal Volume: 67; Journal Issue: (6) ; 06, 2011; Journal ID: ISSN 0907-4449
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; AUTOMATION; CRYSTALLIZATION; DATA ACQUISITION; DIFFRACTION; ELECTRON DENSITY; MODIFICATIONS; PIPELINES; PLATES; PROTEINS; SYNCHROTRONS; X-RAY DIFFRACTION

Citation Formats

Kisselman, Gera, Qiu, Wei, Romanov, Vladimir, Thompson, Christine M., Lam, Robert, Battaile, Kevin P., Pai, Emil F., Chirgadze, Nickolay Y., OCI), and HWMRI). X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection. United States: N. p., 2011. Web. doi:10.1107/S0907444911011589.
Kisselman, Gera, Qiu, Wei, Romanov, Vladimir, Thompson, Christine M., Lam, Robert, Battaile, Kevin P., Pai, Emil F., Chirgadze, Nickolay Y., OCI), & HWMRI). X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection. United States. doi:10.1107/S0907444911011589.
Kisselman, Gera, Qiu, Wei, Romanov, Vladimir, Thompson, Christine M., Lam, Robert, Battaile, Kevin P., Pai, Emil F., Chirgadze, Nickolay Y., OCI), and HWMRI). Mon . "X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection". United States. doi:10.1107/S0907444911011589.
@article{osti_1020578,
title = {X-CHIP: an integrated platform for high-throughput protein crystallization and on-the-chip X-ray diffraction data collection},
author = {Kisselman, Gera and Qiu, Wei and Romanov, Vladimir and Thompson, Christine M. and Lam, Robert and Battaile, Kevin P. and Pai, Emil F. and Chirgadze, Nickolay Y. and OCI) and HWMRI)},
abstractNote = {The X-CHIP (X-ray Crystallization High-throughput Integrated Platform) is a novel microchip that has been developed to combine multiple steps of the crystallographic pipeline from crystallization to diffraction data collection on a single device to streamline the entire process. The system has been designed for crystallization condition screening, visual crystal inspection, initial X-ray screening and data collection in a high-throughput fashion. X-ray diffraction data acquisition can be performed directly on-the-chip at room temperature using an in situ approach. The capabilities of the chip eliminate the necessity for manual crystal handling and cryoprotection of crystal samples, while allowing data collection from multiple crystals in the same drop. This technology would be especially beneficial for projects with large volumes of data, such as protein-complex studies and fragment-based screening. The platform employs hydrophilic and hydrophobic concentric ring surfaces on a miniature plate transparent to visible light and X-rays to create a well defined and stable microbatch crystallization environment. The results of crystallization and data-collection experiments demonstrate that high-quality well diffracting crystals can be grown and high-resolution diffraction data sets can be collected using this technology. Furthermore, the quality of a single-wavelength anomalous dispersion data set collected with the X-CHIP at room temperature was sufficient to generate interpretable electron-density maps. This technology is highly resource-efficient owing to the use of nanolitre-scale drop volumes. It does not require any modification for most in-house and synchrotron beamline systems and offers a promising opportunity for full automation of the X-ray structure-determination process.},
doi = {10.1107/S0907444911011589},
journal = {Acta Crystallogr. D},
issn = {0907-4449},
number = (6) ; 06, 2011,
volume = 67,
place = {United States},
year = {2011},
month = {7}
}