Methods for analyzing nucleic acid sequences

Korlach, Jonas; Webb, Watt W; Levene, Michael; Turner, Stephen; Craighead, Harold G; Foquet, Mathieu

Methods for analyzing nucleic acid sequences

Patent · 17 May 2011

OSTI ID:1020255

Korlach, Jonas ^[1]; Webb, Watt W ^[1]; Levene, Michael ^[1]; Turner, Stephen ^[1]; Craighead, Harold G ^[1]; Foquet, Mathieu ^[1]

Ithaca, NY

The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.

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Research Organization:: Cornell Research Foundation (Ithaca, NY)

Sponsoring Organization:: USDOE

Assignee:: Cornell Research Foundation (Ithaca, NY)

Patent Number(s):: 7,943,307

Application Number:: 11/336,122

OSTI ID:: 1020255

Country of Publication:: United States

Language:: English

References (28)

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