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Title: Hyperaccumulator Alyssum murale Relies on a Different Metal Storage Mechanism for Cobalt than for Nickel

Abstract

The nickel (Ni) hyperaccumulator Alyssum murale has been developed as a commercial crop for phytoremediation/phytomining Ni from metal-enriched soils. Here, metal co-tolerance, accumulation and localization were investigated for A. murale exposed to metal co-contaminants. A. murale was irrigated with Ni-enriched nutrient solutions containing basal or elevated concentrations of cobalt (Co) or zinc (Zn). Metal localization and elemental associations were investigated in situ with synchrotron X-ray microfluorescence (SXRF) and computed-microtomography (CMT). A. murale hyperaccumulated Ni and Co (> 1000 {micro}g g{sup -1} dry weight) from mixed-metal systems. Zinc was not hyperaccumulated. Elevated Co or Zn concentrations did not alter Ni accumulation or localization. SXRF images showed uniform Ni distribution in leaves and preferential localization of Co near leaf tips/margins. CMT images revealed that leaf epidermal tissue was enriched with Ni but devoid of Co, that Co was localized in the apoplasm of leaf ground tissue and that Co was sequestered on leaf surfaces near the tips/margins. Cobalt-rich mineral precipitate(s) form on leaves of Co-treated A. murale. Specialized biochemical processes linked with Ni (hyper)tolerance in A. murale do not confer (hyper)tolerance to Co. A. murale relies on a different metal storage mechanism for Co (exocellular sequestration) than for Ni (vacuolar sequestration).

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL) National Synchrotron Light Source
Sponsoring Org.:
DOE - OFFICE OF SCIENCE
OSTI Identifier:
1020247
Report Number(s):
BNL-96097-2011-JA
Journal ID: ISSN 0028-646X; NEPHAV; TRN: US201116%%227
DOE Contract Number:
DE-AC02-98CH10886
Resource Type:
Journal Article
Resource Relation:
Journal Name: New Phytologist; Journal Volume: 175; Journal Issue: 4
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; COBALT; CROPS; DISTRIBUTION; NICKEL; NUTRIENTS; SOILS; STORAGE; SYNCHROTRONS; TOLERANCE; ZINC; national synchrotron light source

Citation Formats

Tappero, R., Peltier, E, Grafe, M, Heidel, K, Ginder-Vogel, M, Livi, K, Rivers, M, Marcus, M, Chaney, R, and Sparks, D. Hyperaccumulator Alyssum murale Relies on a Different Metal Storage Mechanism for Cobalt than for Nickel. United States: N. p., 2007. Web. doi:10.1111/j.1469-8137.2007.02134.x.
Tappero, R., Peltier, E, Grafe, M, Heidel, K, Ginder-Vogel, M, Livi, K, Rivers, M, Marcus, M, Chaney, R, & Sparks, D. Hyperaccumulator Alyssum murale Relies on a Different Metal Storage Mechanism for Cobalt than for Nickel. United States. doi:10.1111/j.1469-8137.2007.02134.x.
Tappero, R., Peltier, E, Grafe, M, Heidel, K, Ginder-Vogel, M, Livi, K, Rivers, M, Marcus, M, Chaney, R, and Sparks, D. Mon . "Hyperaccumulator Alyssum murale Relies on a Different Metal Storage Mechanism for Cobalt than for Nickel". United States. doi:10.1111/j.1469-8137.2007.02134.x.
@article{osti_1020247,
title = {Hyperaccumulator Alyssum murale Relies on a Different Metal Storage Mechanism for Cobalt than for Nickel},
author = {Tappero, R. and Peltier, E and Grafe, M and Heidel, K and Ginder-Vogel, M and Livi, K and Rivers, M and Marcus, M and Chaney, R and Sparks, D},
abstractNote = {The nickel (Ni) hyperaccumulator Alyssum murale has been developed as a commercial crop for phytoremediation/phytomining Ni from metal-enriched soils. Here, metal co-tolerance, accumulation and localization were investigated for A. murale exposed to metal co-contaminants. A. murale was irrigated with Ni-enriched nutrient solutions containing basal or elevated concentrations of cobalt (Co) or zinc (Zn). Metal localization and elemental associations were investigated in situ with synchrotron X-ray microfluorescence (SXRF) and computed-microtomography (CMT). A. murale hyperaccumulated Ni and Co (> 1000 {micro}g g{sup -1} dry weight) from mixed-metal systems. Zinc was not hyperaccumulated. Elevated Co or Zn concentrations did not alter Ni accumulation or localization. SXRF images showed uniform Ni distribution in leaves and preferential localization of Co near leaf tips/margins. CMT images revealed that leaf epidermal tissue was enriched with Ni but devoid of Co, that Co was localized in the apoplasm of leaf ground tissue and that Co was sequestered on leaf surfaces near the tips/margins. Cobalt-rich mineral precipitate(s) form on leaves of Co-treated A. murale. Specialized biochemical processes linked with Ni (hyper)tolerance in A. murale do not confer (hyper)tolerance to Co. A. murale relies on a different metal storage mechanism for Co (exocellular sequestration) than for Ni (vacuolar sequestration).},
doi = {10.1111/j.1469-8137.2007.02134.x},
journal = {New Phytologist},
number = 4,
volume = 175,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2007},
month = {Mon Jan 01 00:00:00 EST 2007}
}