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Peptide identification

Patent ·
OSTI ID:1019425
 [1];  [1];  [1];  [1]
  1. Richland, WA
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Peptides are identified from a list of candidates using collision-induced dissociation tandem mass spectrometry data. A probabilistic model for the occurrence of spectral peaks corresponding to frequently observed partial peptide fragment ions is applied. As part of the identification procedure, a probability score is produced that indicates the likelihood of any given candidate being the correct match. The statistical significance of the score is known without necessarily having reference to the actual identity of the peptide. In one form of the invention, a genetic algorithm is applied to candidate peptides using an objective function that takes into account the number of shifted peaks appearing in the candidate spectrum relative to the test spectrum.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
7,979,214
Application Number:
US Patent Application 11/592,610
OSTI ID:
1019425
Country of Publication:
United States
Language:
English

References (13)

ProFound:  An Expert System for Protein Identification Using Mass Spectrometric Peptide Mapping Information journal June 2000
An Algorithm for Automated Bacterial Identification Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry journal March 2000
Efficiency of Database Search for Identification of Mutated and Modified Proteins via Mass Spectrometry journal February 2001
Role of Accurate Mass Measurement (±10 ppm) in Protein Identification Strategies Employing MS or MS/MS and Database Searching journal July 1999
De Novo Peptide Sequencing via Tandem Mass Spectrometry journal October 1999
Sequence optimization as an alternative to de novo analysis of tandem mass spectrometry data journal April 2004
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database journal November 1994
Analysis of Microbial Mixtures by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry journal December 2002
SCOPE: a probabilistic model for scoring tandem mass spectra against a peptide database journal June 2001
Mutation-Tolerant Protein Identification by Mass Spectrometry journal December 2000
Probability-based protein identification by searching sequence databases using mass spectrometry data journal December 1999
Scoring methods in MALDI peptide mass fingerprinting: ChemScore, and the ChemApplex program journal January 2002
Use of Artificial Neural Networks for the Accurate Prediction of Peptide Liquid Chromatography Elution Times in Proteome Analyses journal March 2003

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