Genomic library construction

Church, George M; Zhang, Kun

Genomic library construction

Patent · 26 July 2011

OSTI ID:1019402

Church, George M ^[1]; Zhang, Kun ^[2]

Brookline, MA
San Diego, CA

Compositions and methods for amplifying nucleic acid sequences from a single cell are provided. Compositions and methods for constructing a genomic library from a single cell are also provided.

View Patent

Research Organization:: Harvard University

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-02ER63445

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Number(s):: 7,985,546

Application Number:: US Patent Application 12/265,184

OSTI ID:: 1019402

Country of Publication:: United States

Language:: English

References (17)

Mutation detection and single-molecule counting using isothermal rolling-circleamplification Lizardi, Paul M.; Huang, Xiaohua; Zhu, Zhengrong Nature Genetics, Vol. 19, Issue 3, p. 225-232 https://doi.org/10.1038/898	journal	July 1998
Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer Telenius, Ha˚kan; Carter, Nigel P.; Bebb, Charlotte E. Genomics, Vol. 13, Issue 3 https://doi.org/10.1016/0888-7543(92)90147-K	journal	July 1992
Whole genome amplification from a single cell: implications for genetic analysis. Zhang, L.; Cui, X.; Schmitt, K. Proceedings of the National Academy of Sciences, Vol. 89, Issue 13 https://doi.org/10.1073/pnas.89.13.5847	journal	July 1992
Cell-free cloning using φ29 DNA polymerase Hutchison, C. A.; Smith, H. O.; Pfannkoch, C. Proceedings of the National Academy of Sciences, Vol. 102, Issue 48, p. 17332-17336 https://doi.org/10.1073/pnas.0508809102	journal	November 2005
Fine-scale phylogenetic architecture of a complex bacterial community Acinas, Silvia G.; Klepac-Ceraj, Vanja; Hunt, Dana E. Nature, Vol. 430, Issue 6999 https://doi.org/10.1038/nature02649	journal	July 2004
Genome coverage and sequence fidelity of 29 polymerase-based multiple strand displacement whole genome amplification Paez, J. G. Nucleic Acids Research, Vol. 32, Issue 9 https://doi.org/10.1093/nar/gnh069	journal	May 2004
Ligation overcomes terminal underrepresentation in multiple displacement amplification of linear DNA Panelli, Simona; Damiani, Giuseppe; Espen, Luca BioTechniques, Vol. 39, Issue 2 https://doi.org/10.2144/05392BM03	journal	August 2005
Comprehensive human genome amplification using multiple displacement amplification Dean, F. B.; Hosono, S.; Fang, L. Proceedings of the National Academy of Sciences, Vol. 99, Issue 8, p. 5261-5266 https://doi.org/10.1073/pnas.082089499	journal	April 2002
Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome Shendure, J. Science, Vol. 309, Issue 5741 https://doi.org/10.1126/science.1117389	journal	September 2005
Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase Hafner, G. J.; Yang, I. C.; Wolter, L. C. BioTechniques, Vol. 30, Issue 4 https://doi.org/10.2144/01304rr03	journal	April 2001
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. Walker, G. T.; Little, M. C.; Nadeau, J. G. Proceedings of the National Academy of Sciences, Vol. 89, Issue 1, p. 392-396 https://doi.org/10.1073/pnas.89.1.392	journal	January 1992
Unbiased Whole-Genome Amplification Directly From Clinical Samples Hosono, S. Genome Research, Vol. 13, Issue 5 https://doi.org/10.1101/gr.816903	journal	April 2003
Genotypic Diversity Within a Natural Coastal Bacterioplankton Population Thompson, J. R. Science, Vol. 307, Issue 5713 https://doi.org/10.1126/science.1106028	journal	February 2005
Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array-CGH Lage, J. M. Genome Research, Vol. 13, Issue 2 https://doi.org/10.1101/gr.377203	journal	February 2003
Advanced sequencing technologies: methods and goals Shendure, Jay; Mitra, Robi D.; Varma, Chris Nature Reviews Genetics, Vol. 5, Issue 5 https://doi.org/10.1038/nrg1325	journal	May 2004
Genomic DNA Amplification from a Single Bacterium Raghunathan, A.; Ferguson, H. R.; Bornarth, C. J. Applied and Environmental Microbiology, Vol. 71, Issue 6 https://doi.org/10.1128/AEM.71.6.3342-3347.2005	journal	June 2005
Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics Detter, John C.; Jett, Jamie M.; Lucas, Susan M. Genomics, Vol. 80, Issue 6 https://doi.org/10.1006/geno.2002.7020	journal	December 2002

Similar Records

Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs

Patent · 1998 · OSTI ID:321194

Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs

Patent · 1997 · OSTI ID:872025

Frequency-based modulation of diverse species in a nucleic acid library

Patent · 2020 · OSTI ID:1771684

Related Subjects

47 OTHER INSTRUMENTATION

Genomic library construction

Citation Formats

References (17)

Similar Records

Related Subjects