skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol

Abstract

The invention provides for a method for producing a 5-carbon alcohol in a genetically modified host cell. In one embodiment, the method comprises culturing a genetically modified host cell which expresses a first enzyme capable of catalyzing the dephosphorylation of an isopentenyl pyrophosphate (IPP) or dimethylallyl diphosphate (DMAPP), such as a Bacillus subtilis phosphatase (YhfR), under a suitable condition so that 5-carbon alcohol is 3-methyl-2-buten-1-ol and/or 3-methyl-3-buten-1-ol is produced. Optionally, the host cell may further comprise a second enzyme capable of reducing a 3-methyl-2-buten-1-ol to 3-methyl-butan-1-ol, such as a reductase.

Inventors:
 [1];  [1]
  1. Berkeley, CA
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1019401
Patent Number(s):
7,985,567
Application Number:
US Patent Application 12/646,189
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Chou, Howard H, and Keasling, Jay D. Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol. United States: N. p., 2011. Web.
Chou, Howard H, & Keasling, Jay D. Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol. United States.
Chou, Howard H, and Keasling, Jay D. Tue . "Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol". United States. https://www.osti.gov/servlets/purl/1019401.
@article{osti_1019401,
title = {Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol},
author = {Chou, Howard H and Keasling, Jay D},
abstractNote = {The invention provides for a method for producing a 5-carbon alcohol in a genetically modified host cell. In one embodiment, the method comprises culturing a genetically modified host cell which expresses a first enzyme capable of catalyzing the dephosphorylation of an isopentenyl pyrophosphate (IPP) or dimethylallyl diphosphate (DMAPP), such as a Bacillus subtilis phosphatase (YhfR), under a suitable condition so that 5-carbon alcohol is 3-methyl-2-buten-1-ol and/or 3-methyl-3-buten-1-ol is produced. Optionally, the host cell may further comprise a second enzyme capable of reducing a 3-methyl-2-buten-1-ol to 3-methyl-butan-1-ol, such as a reductase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {7}
}

Patent:

Save / Share:

Works referenced in this record:

A Study of the Structure–Octane Number Relationship for Hydrocarbons
journal, July 2004


Reductive biotransformations of organic compounds by cells or enzymes of yeast
journal, July 1990


Genome-wide Analysis of Substrate Specificities of the Escherichia coli Haloacid Dehalogenase-like Phosphatase Family
journal, September 2006

  • Kuznetsova, Ekaterina; Proudfoot, Michael; Gonzalez, Claudio F.
  • Journal of Biological Chemistry, Vol. 281, Issue 47
  • DOI: 10.1074/jbc.M605449200

A Soluble Form of Phosphatase in Saccharomyces cerevisiae Capable of Converting Farnesyl Diphosphate Into E-E Farnesol
journal, January 2006

  • Song, Linsheng
  • Applied Biochemistry and Biotechnology, Vol. 128, Issue 2, p. 149-158
  • DOI: 10.1385/ABAB:128:2:149

The Nudix hydrolase superfamily
journal, December 2005


Effects of overproduction of the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase on squalene synthesis in Saccharomyces cerevisiae.
journal, January 1997


A cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus is actually a broad specificity phosphatase
journal, September 2001

  • Rigden, Daniel J.; Bagyan, Irina; Lamani, Ejvis
  • Protein Science, Vol. 10, Issue 9
  • DOI: 10.1110/ps.15701

Toxicity Caused by Hydroxycinnamoyl-Coenzyme A Thioester Accumulation in Mutants of Acinetobacter sp. Strain ADP1
journal, May 2004


Overproduction of a Functional Fatty Acid Biosynthetic Enzyme Blocks Fatty Acid Synthesis inEscherichia coli
journal, September 1998


Identification, Evolution, and Essentiality of the Mevalonate Pathway for Isopentenyl Diphosphate Biosynthesis in Gram-Positive Cocci
journal, August 2000


The activity of yeast ADH I and ADH II with long-chain alcohols and diols
journal, January 2001


Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast
journal, January 1998

  • Polakowski, T.; Stahl, U.; Lang, C.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 1
  • DOI: 10.1007/s002530051138

Phosphoryl group transfer: evolution of a catalytic scaffold
journal, September 2004


Metabolic Engineering to Produce Sesquiterpenes in Yeast
journal, May 2003

  • Jackson, Beth E.; Hart-Wells, Elizabeth A.; Matsuda, Seiichi P. T.
  • Organic Letters, Vol. 5, Issue 10
  • DOI: 10.1021/ol034231x

Production of the antimalarial drug precursor artemisinic acid in engineered yeast
journal, April 2006

  • Ro, Dae-Kyun; Paradise, Eric M.; Ouellet, Mario
  • Nature, Vol. 440, Issue 7086, p. 940-943
  • DOI: 10.1038/nature04640

High-Level Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of Recombinant Escherichia coli
journal, October 1999


Structures and mechanisms of Nudix hydrolases
journal, January 2005

  • Mildvan, A. S.; Xia, Z.; Azurmendi, H. F.
  • Archives of Biochemistry and Biophysics, Vol. 433, Issue 1
  • DOI: 10.1016/j.abb.2004.08.017

Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production
journal, August 2003

  • Murli, Sumati; Kennedy, Jonathan; Dayem, Linda C.
  • Journal of Industrial Microbiology and Biotechnology, Vol. 30, Issue 8
  • DOI: 10.1007/s10295-003-0073-x

Mono and diterpene production in Escherichia coli
journal, January 2004

  • Reiling, K. Kinkead; Yoshikuni, Yasuo; Martin, Vincent J.J.
  • Biotechnology and Bioengineering, Vol. 87, Issue 2, p. 200-212
  • DOI: 10.1002/bit.20128

Cloning of a Gene Cluster Encoding Enzymes Responsible for the Mevalonate Pathway from a Terpenoid-antibiotic-producing Streptomyces Strain
journal, January 2001

  • Hamano, Yoshimitsu; Dairi, Tohru; Yamamoto, Masahiro
  • Bioscience, Biotechnology, and Biochemistry, Vol. 65, Issue 7
  • DOI: 10.1271/bbb.65.1627

Highly Stereoselective Reagents for β-Keto Ester Reductions by Genetic Engineering of Baker's Yeast
journal, February 2001

  • Rodríguez, Sonia; Kayser, Margaret M.; Stewart, Jon D.
  • Journal of the American Chemical Society, Vol. 123, Issue 8
  • DOI: 10.1021/ja0027968

‘New uses for an Old Enzyme’ – the Old Yellow Enzyme family of flavoenzymes
journal, June 2002


Identification of Isopentenol Biosynthetic Genes from Bacillus subtilis by a Screening Method Based on Isoprenoid Precursor Toxicity
journal, August 2007

  • Withers, S. T.; Gottlieb, S. S.; Lieu, B.
  • Applied and Environmental Microbiology, Vol. 73, Issue 19, p. 6277-6283
  • DOI: 10.1128/AEM.00861-07

Production of mevalonate by a metabolically-engineered Escherichia coli
journal, October 2004


On the mechanism of action of the antifungal agent propionate: Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans
journal, July 2004


Heterologous Mevalonate Production in Streptomyces lividans TK23
journal, January 2004

  • Kuzuyama, Tomohisa; Dairi, Tohru; Yamashita, Haruyuki
  • Bioscience, Biotechnology, and Biochemistry, Vol. 68, Issue 4
  • DOI: 10.1271/bbb.68.931

Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase fromBacillus subtilis
journal, July 2000


Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
journal, June 2003

  • Martin, Vincent J. J.; Pitera, Douglas J.; Withers, Sydnor T.
  • Nature Biotechnology, Vol. 21, Issue 7
  • DOI: 10.1038/nbt833