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Title: Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici

Abstract

Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101 mega base pair genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89 mega base pair genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,841 predicted proteins of M. larici-populina to the 18,241 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic life-style include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins (SSPs), impaired nitrogen and sulfur assimilation pathways, and expanded families of amino-acid, oligopeptide and hexose membrane transporters. The dramatic upregulation of transcripts coding for SSPs, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergentmore » adaptation to a biotrophic existence inside plant cells« less

Authors:
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Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
Genomics Division
OSTI Identifier:
1019309
Report Number(s):
LBNL-4586E
Journal ID: ISSN 0027--8424; TRN: US201114%%750
DOE Contract Number:  
DE-AC02-05CH11231
Resource Type:
Journal Article
Journal Name:
PNAS
Additional Journal Information:
Journal Volume: 108; Journal Issue: 22; Journal ID: ISSN 0027--8424
Country of Publication:
United States
Language:
English
Subject:
99; AVOIDANCE; BARLEY; CROPS; ENZYMES; FUNGI; GENES; HEXOSES; IMMUNITY; MEMBRANES; NITROGEN; NUTRIENTS; PATHOGENS; PLANT CELLS; PLANT TISSUES; POPLARS; PROTEINS; SULFUR; WHEAT; Rust Fungi, melampsora larici-populina, Puccinia graminis

Citation Formats

Duplessis, Sebastien, Cuomo, Christina A, Lin, Yao-Cheng, Aerts, Andrea, Tisserant, Emilie, Veneault-Fourrey, Claire, Joly, David L, Hacquard, Stephane, Amselem, Joelle, Cantarel, Brandi, Chiu, Readman, Couthinho, Pedro, Feau, Nicolas, Field, Matthew, Frey, Pascal, Gelhaye, Eric, Goldberg, Jonathan, Grabherr, Manfred, Kodira, Chinnappa, Kohler, Annegret, Kues, Ursula, Lindquist, Erika, Lucas, Susan, Mago, Rohit, Mauceli, Evan, Morin, Emmanuelle, Murat, Claude, Pangilinan, Jasmyn L, Park, Robert, Pearson, Matthew, Quesneville, Hadi, Rouhier, Nicolas, Sakthikumar, Sharadha, Salamov, Asaf A, Schmutz, Jeremy, Selles, Benjamin, Shapiro, Harris, Tangay, Philippe, Tuskan, Gerald A, Peer, Yves Van de, Henrissat, Bernard, Rouze, Pierre, Ellis, Jeffrey G, Dodds, Peter N, Schein, Jacqueline E, Zhong, Shaobin, Hamelin, Richard C, Grigoriev, Igor V, Szabo, Les J, and Martin1, Francis. Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici. United States: N. p., 2011. Web. doi:10.1073/pnas.1019315108.
Duplessis, Sebastien, Cuomo, Christina A, Lin, Yao-Cheng, Aerts, Andrea, Tisserant, Emilie, Veneault-Fourrey, Claire, Joly, David L, Hacquard, Stephane, Amselem, Joelle, Cantarel, Brandi, Chiu, Readman, Couthinho, Pedro, Feau, Nicolas, Field, Matthew, Frey, Pascal, Gelhaye, Eric, Goldberg, Jonathan, Grabherr, Manfred, Kodira, Chinnappa, Kohler, Annegret, Kues, Ursula, Lindquist, Erika, Lucas, Susan, Mago, Rohit, Mauceli, Evan, Morin, Emmanuelle, Murat, Claude, Pangilinan, Jasmyn L, Park, Robert, Pearson, Matthew, Quesneville, Hadi, Rouhier, Nicolas, Sakthikumar, Sharadha, Salamov, Asaf A, Schmutz, Jeremy, Selles, Benjamin, Shapiro, Harris, Tangay, Philippe, Tuskan, Gerald A, Peer, Yves Van de, Henrissat, Bernard, Rouze, Pierre, Ellis, Jeffrey G, Dodds, Peter N, Schein, Jacqueline E, Zhong, Shaobin, Hamelin, Richard C, Grigoriev, Igor V, Szabo, Les J, & Martin1, Francis. Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici. United States. https://doi.org/10.1073/pnas.1019315108
Duplessis, Sebastien, Cuomo, Christina A, Lin, Yao-Cheng, Aerts, Andrea, Tisserant, Emilie, Veneault-Fourrey, Claire, Joly, David L, Hacquard, Stephane, Amselem, Joelle, Cantarel, Brandi, Chiu, Readman, Couthinho, Pedro, Feau, Nicolas, Field, Matthew, Frey, Pascal, Gelhaye, Eric, Goldberg, Jonathan, Grabherr, Manfred, Kodira, Chinnappa, Kohler, Annegret, Kues, Ursula, Lindquist, Erika, Lucas, Susan, Mago, Rohit, Mauceli, Evan, Morin, Emmanuelle, Murat, Claude, Pangilinan, Jasmyn L, Park, Robert, Pearson, Matthew, Quesneville, Hadi, Rouhier, Nicolas, Sakthikumar, Sharadha, Salamov, Asaf A, Schmutz, Jeremy, Selles, Benjamin, Shapiro, Harris, Tangay, Philippe, Tuskan, Gerald A, Peer, Yves Van de, Henrissat, Bernard, Rouze, Pierre, Ellis, Jeffrey G, Dodds, Peter N, Schein, Jacqueline E, Zhong, Shaobin, Hamelin, Richard C, Grigoriev, Igor V, Szabo, Les J, and Martin1, Francis. Wed . "Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici". United States. https://doi.org/10.1073/pnas.1019315108. https://www.osti.gov/servlets/purl/1019309.
@article{osti_1019309,
title = {Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici},
author = {Duplessis, Sebastien and Cuomo, Christina A and Lin, Yao-Cheng and Aerts, Andrea and Tisserant, Emilie and Veneault-Fourrey, Claire and Joly, David L and Hacquard, Stephane and Amselem, Joelle and Cantarel, Brandi and Chiu, Readman and Couthinho, Pedro and Feau, Nicolas and Field, Matthew and Frey, Pascal and Gelhaye, Eric and Goldberg, Jonathan and Grabherr, Manfred and Kodira, Chinnappa and Kohler, Annegret and Kues, Ursula and Lindquist, Erika and Lucas, Susan and Mago, Rohit and Mauceli, Evan and Morin, Emmanuelle and Murat, Claude and Pangilinan, Jasmyn L and Park, Robert and Pearson, Matthew and Quesneville, Hadi and Rouhier, Nicolas and Sakthikumar, Sharadha and Salamov, Asaf A and Schmutz, Jeremy and Selles, Benjamin and Shapiro, Harris and Tangay, Philippe and Tuskan, Gerald A and Peer, Yves Van de and Henrissat, Bernard and Rouze, Pierre and Ellis, Jeffrey G and Dodds, Peter N and Schein, Jacqueline E and Zhong, Shaobin and Hamelin, Richard C and Grigoriev, Igor V and Szabo, Les J and Martin1, Francis},
abstractNote = {Rust fungi are some of the most devastating pathogens of crop plants. They are obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart from their hosts. Their lifestyle has slowed the dissection of molecular mechanisms underlying host invasion and avoidance or suppression of plant innate immunity. We sequenced the 101 mega base pair genome of Melampsora larici-populina, the causal agent of poplar leaf rust, and the 89 mega base pair genome of Puccinia graminis f. sp. tritici, the causal agent of wheat and barley stem rust. We then compared the 16,841 predicted proteins of M. larici-populina to the 18,241 predicted proteins of P. graminis f. sp tritici. Genomic features related to their obligate biotrophic life-style include expanded lineage-specific gene families, a large repertoire of effector-like small secreted proteins (SSPs), impaired nitrogen and sulfur assimilation pathways, and expanded families of amino-acid, oligopeptide and hexose membrane transporters. The dramatic upregulation of transcripts coding for SSPs, secreted hydrolytic enzymes, and transporters in planta suggests that they play a role in host infection and nutrient acquisition. Some of these genomic hallmarks are mirrored in the genomes of other microbial eukaryotes that have independently evolved to infect plants, indicating convergent adaptation to a biotrophic existence inside plant cells},
doi = {10.1073/pnas.1019315108},
url = {https://www.osti.gov/biblio/1019309}, journal = {PNAS},
issn = {0027--8424},
number = 22,
volume = 108,
place = {United States},
year = {2011},
month = {4}
}