Nucleic acid encoding a self-assembling split-fluorescent protein system

Waldo, Geoffrey S; Cabantous, Stephanie

Title: Nucleic acid encoding a self-assembling split-fluorescent protein system

Patent · Tue Jun 07 00:00:00 EDT 2011

OSTI ID:1019114

Waldo, Geoffrey S ^[1]; Cabantous, Stephanie ^[2]

Santa Fe, NM
Los Alamos, NM

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-98ER62647; W-7405-ENG-36

Assignee:: Los Alamos National Security, LLC (Los Alamos, NM)

Patent Number(s):: 7,955,821

Application Number:: US Patent Application 10/973,693

OSTI ID:: 1019114

Country of Publication:: United States

Language:: English

References (26)

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