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Title: Development of novel separation techniques for biological samples in capillary electrophoresis

Abstract

This dissertation includes three different topics: general introduction of capillary electrophoresis (CE); gradient in CE and CE in biological separations; and capillary gel electrophoresis (CGE) for DNA separation. Factors such as temperature, viscosity, pH, and the surface of capillary walls affecting the separation performance are demonstrated. A pH gradient between 3.0 and 5.2 is useful to improve the resolution among eight different organic acids. A flow gradient due to the change in the concentration of surfactant, which is able to coat to the capillary wall to change the flow rate and its direction, is also shown as a good way to improve the resolution for organic compounds. A temperature gradient caused by joule heat is shown by voltage programming to enhance the resolution and shorten the separation time for several phenolic compounds. The author also shows that self-regulating dynamic control of electroosmotic flow in CE by simply running separation in different concentrations of surfactant has less matrix effect on the separation performance. One of the most important demonstrations in this dissertation is that the author proposes on-column reaction which gives several advantages including the use of a small amount of sample, low risk of contamination, and time saving and kineticmore » features. The author uses this idea with laser induced fluorescence (LIF) as a detection mode to detect an on-column digestion of sub-ng of protein. This technique also is applied to single cell analysis in the group.« less

Authors:
 [1]
  1. Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Iowa State Univ. of Science and Technology, Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
10190677
Report Number(s):
IS-T-1710
ON: DE95001672; TRN: AHC29427%%64
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: TH: Thesis (Ph.D.); PBD: 27 Jul 1994
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ELECTROPHORESIS; PERFORMANCE; PROTEINS; DNA; ORGANIC COMPOUNDS; SEPARATION PROCESSES; OPTIMIZATION; LASER RADIATION; 550200; 400105; BIOCHEMISTRY; SEPARATION PROCEDURES

Citation Formats

Chang, Huan -Tsung. Development of novel separation techniques for biological samples in capillary electrophoresis. United States: N. p., 1994. Web. doi:10.2172/10190677.
Chang, Huan -Tsung. Development of novel separation techniques for biological samples in capillary electrophoresis. United States. doi:10.2172/10190677.
Chang, Huan -Tsung. Wed . "Development of novel separation techniques for biological samples in capillary electrophoresis". United States. doi:10.2172/10190677. https://www.osti.gov/servlets/purl/10190677.
@article{osti_10190677,
title = {Development of novel separation techniques for biological samples in capillary electrophoresis},
author = {Chang, Huan -Tsung},
abstractNote = {This dissertation includes three different topics: general introduction of capillary electrophoresis (CE); gradient in CE and CE in biological separations; and capillary gel electrophoresis (CGE) for DNA separation. Factors such as temperature, viscosity, pH, and the surface of capillary walls affecting the separation performance are demonstrated. A pH gradient between 3.0 and 5.2 is useful to improve the resolution among eight different organic acids. A flow gradient due to the change in the concentration of surfactant, which is able to coat to the capillary wall to change the flow rate and its direction, is also shown as a good way to improve the resolution for organic compounds. A temperature gradient caused by joule heat is shown by voltage programming to enhance the resolution and shorten the separation time for several phenolic compounds. The author also shows that self-regulating dynamic control of electroosmotic flow in CE by simply running separation in different concentrations of surfactant has less matrix effect on the separation performance. One of the most important demonstrations in this dissertation is that the author proposes on-column reaction which gives several advantages including the use of a small amount of sample, low risk of contamination, and time saving and kinetic features. The author uses this idea with laser induced fluorescence (LIF) as a detection mode to detect an on-column digestion of sub-ng of protein. This technique also is applied to single cell analysis in the group.},
doi = {10.2172/10190677},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {7}
}

Thesis/Dissertation:
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