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Title: Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization

Abstract

A novel hydrodynamic injector that is directly controlled by a pneumatic valve has been developed for reproducible microchip capillary electrophoresis (CE) separations. The poly(dimethylsiloxane) (PDMS) devices used for evaluation comprise a separation channel, a side channel for sample introduction, and a pneumatic valve aligned at the intersection of the channels. A low pressure (≤ 3 psi) applied to the sample reservoir is sufficient to drive sample into the separation channel. The rapidly actuated pneumatic valve enables injection of discrete sample plugs as small as ~100 pL for CE separation. The injection volume can be easily controlled by adjusting the intersection geometry, the solution back pressure and the valve actuation time. Sample injection could be reliably operated at different frequencies (< 0.1 Hz to >2 Hz) with good reproducibility (peak height relative standard deviation ≤ 3.6%) and no sampling biases associated with the conventional electrokinetic injections. The separation channel was dynamically coated with a cationic polymer, and FITC-labeled amino acids were employed to evaluate the CE separation. Highly efficient (≥ 7.0 × 103 theoretical plates for the ~2.4 cm long channel) and reproducible CE separations were obtained. The demonstrated method has numerous advantages compared with the conventional techniques, including repeatable andmore » unbiased injections, no sample waste, high duty cycle, controllable injected sample volume, and fewer electrodes with no need for voltage switching. The prospects of implementing this injection method for coupling multidimensional separations, for multiplexing CE separations and for sample-limited bioanalyses are discussed.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1018140
Report Number(s):
PNNL-SA-76945
20492a; 400412000; TRN: US201113%%578
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Electrophoresis, 32(13):1610-1618
Additional Journal Information:
Journal Volume: 32; Journal Issue: 13
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; AMINO ACIDS; ELECTRODES; ELECTRODYNAMICS; ELECTROPHORESIS; EVALUATION; GEOMETRY; HYDRODYNAMICS; PLATES; PNEUMATICS; SAMPLING; VALVES; Hydrodynamic injection; Microchip electrophoresis; Microfluidics; Pneumatic valve; Repeatable injection; Environmental Molecular Sciences Laboratory

Citation Formats

Sun, Xuefei, Kelly, Ryan T, Danielson, William F, Agrawal, Nitin, Tang, Keqi, and Smith, Richard D. Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization. United States: N. p., 2011. Web. doi:10.1002/elps.201000522.
Sun, Xuefei, Kelly, Ryan T, Danielson, William F, Agrawal, Nitin, Tang, Keqi, & Smith, Richard D. Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization. United States. https://doi.org/10.1002/elps.201000522
Sun, Xuefei, Kelly, Ryan T, Danielson, William F, Agrawal, Nitin, Tang, Keqi, and Smith, Richard D. 2011. "Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization". United States. https://doi.org/10.1002/elps.201000522.
@article{osti_1018140,
title = {Hydrodynamic injection with pneumatic valving for microchip electrophoresis with total analyte utilization},
author = {Sun, Xuefei and Kelly, Ryan T and Danielson, William F and Agrawal, Nitin and Tang, Keqi and Smith, Richard D},
abstractNote = {A novel hydrodynamic injector that is directly controlled by a pneumatic valve has been developed for reproducible microchip capillary electrophoresis (CE) separations. The poly(dimethylsiloxane) (PDMS) devices used for evaluation comprise a separation channel, a side channel for sample introduction, and a pneumatic valve aligned at the intersection of the channels. A low pressure (≤ 3 psi) applied to the sample reservoir is sufficient to drive sample into the separation channel. The rapidly actuated pneumatic valve enables injection of discrete sample plugs as small as ~100 pL for CE separation. The injection volume can be easily controlled by adjusting the intersection geometry, the solution back pressure and the valve actuation time. Sample injection could be reliably operated at different frequencies (< 0.1 Hz to >2 Hz) with good reproducibility (peak height relative standard deviation ≤ 3.6%) and no sampling biases associated with the conventional electrokinetic injections. The separation channel was dynamically coated with a cationic polymer, and FITC-labeled amino acids were employed to evaluate the CE separation. Highly efficient (≥ 7.0 × 103 theoretical plates for the ~2.4 cm long channel) and reproducible CE separations were obtained. The demonstrated method has numerous advantages compared with the conventional techniques, including repeatable and unbiased injections, no sample waste, high duty cycle, controllable injected sample volume, and fewer electrodes with no need for voltage switching. The prospects of implementing this injection method for coupling multidimensional separations, for multiplexing CE separations and for sample-limited bioanalyses are discussed.},
doi = {10.1002/elps.201000522},
url = {https://www.osti.gov/biblio/1018140}, journal = {Electrophoresis, 32(13):1610-1618},
number = 13,
volume = 32,
place = {United States},
year = {2011},
month = {4}
}