Analysis of electrospray derived ions using a quadrupole ion trap mass spectrometer
Electrospray (ES) ionization has emerged as a simple and sensitive method by which to produce gas-phase ions from involatile, polar, and thermally labile compounds for analysis by mass spectrometry (MS). Much of the recent interest in ES stems from the ability to produce gas-phase multiply charged ions from high mass biopolymers such as proteins and oligonucleotides. This multiple charging phenomenon effectively extends the mass range of the mass spectrometer and allows for replicate measurements of the molecular weigh thereby increasing the accuracy of the measurement. Moreover, because analytes are sprayed from solution at atmospheric pressure, ES provides a convenient means by which to couple MS and any one of several condensed-phase separation techniques. Another significant advance in MS over the last several years has been the development of the quadrupole ion trap as an analytical mass spectrometer. The ion trap is a small, rugged and relatively low cost mass spectrometer that offers high sensitivity (low detection limits) and the ability to perform high efficiency MS/MS and MS{reg_sign} experiments. These attributes along with the ion trapping nature of the instrument and the more recently demonstrated extended mass-to-charge range and high mass resolution capability make this instrument extremely versatile and well suited as the analyzer for ES derived ions.
- Research Organization:
- Oak Ridge National Lab., TN (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States); Department of Health and Human Services, Washington, DC (United States)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 10180032
- Report Number(s):
- CONF-9209208-1; ON: DE93000979; CNN: Grant R01GM45372
- Resource Relation:
- Conference: 1992 international conference on biological mass spectrometry,Kyoto (Japan),20-24 Sep 1992; Other Information: PBD: [1992]
- Country of Publication:
- United States
- Language:
- English
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