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Title: Highly thermostable fluorescent proteins

Abstract

Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.

Inventors:
 [1];  [1];  [2]
  1. Santa Fe, NM
  2. Los Alamos, NM
Publication Date:
Research Org.:
Los Alamos National Security, LLC (Los Alamos, NM)
Sponsoring Org.:
USDOE
OSTI Identifier:
1016159
Patent Number(s):
7,910,700
Application Number:
US Patent Application 12/317,185
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM) LANL
DOE Contract Number:  
AC52-06NA25396
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bradbury, Andrew M, Waldo, Geoffrey S, and Kiss, Csaba. Highly thermostable fluorescent proteins. United States: N. p., 2011. Web.
Bradbury, Andrew M, Waldo, Geoffrey S, & Kiss, Csaba. Highly thermostable fluorescent proteins. United States.
Bradbury, Andrew M, Waldo, Geoffrey S, and Kiss, Csaba. Tue . "Highly thermostable fluorescent proteins". United States. https://www.osti.gov/servlets/purl/1016159.
@article{osti_1016159,
title = {Highly thermostable fluorescent proteins},
author = {Bradbury, Andrew M and Waldo, Geoffrey S and Kiss, Csaba},
abstractNote = {Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99.degree. C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80.degree. C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {3}
}

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