Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

Simmons, Blake A; Hill, Vincent R; Fintschenko, Yolanda; Cummings, Eric B

Title: Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

Patent · Tue Oct 12 00:00:00 EDT 2010

OSTI ID:1016144

Simmons, Blake A ^[1]; Hill, Vincent R ^[2]; Fintschenko, Yolanda ^[3]; Cummings, Eric B ^[3]

San Francisco, CA
Decatur, GA
Livermore, CA

Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.

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Research Organization:: Sandia Corporation (Livermore, CA)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Livermore, CA)

Patent Number(s):: 7,811,439

Application Number:: 11/499,371

OSTI ID:: 1016144

Country of Publication:: United States

Language:: English

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