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Title: Switchable photosystem-II designer algae for photobiological hydrogen production

Abstract

A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.

Inventors:
 [1]
  1. (Knoxville, TN)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1015190
Patent Number(s):
7,642,405
Application Number:
11/748,550
Assignee:
Lee, James Weifu (Knoxville, TN) ORO
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Lee, James Weifu. Switchable photosystem-II designer algae for photobiological hydrogen production. United States: N. p., 2010. Web.
Lee, James Weifu. Switchable photosystem-II designer algae for photobiological hydrogen production. United States.
Lee, James Weifu. Tue . "Switchable photosystem-II designer algae for photobiological hydrogen production". United States. https://www.osti.gov/servlets/purl/1015190.
@article{osti_1015190,
title = {Switchable photosystem-II designer algae for photobiological hydrogen production},
author = {Lee, James Weifu},
abstractNote = {A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {1}
}

Patent:

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