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Title: Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate

Abstract

Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [6];  [2];  [7]
  1. (Kennett Square, PA)
  2. (Lakewood, CO)
  3. (Centennial, CO)
  4. (Newark, DE)
  5. (Havertown, PA)
  6. (West Chester, PA)
  7. (New Hope, PA)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014558
Patent Number(s):
7,803,623
Application Number:
US Patent Application 12/261,166
Assignee:
E.I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Wilmington, DE) NREL
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Caimi, Perry G., Chou, Yat-Chen, Franden, Mary Ann, Knoke, Kyle, Tao, Luan, Viitanen, Paul V., Zhang, Min, and Zhang, Yuying. Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate. United States: N. p., 2010. Web.
Caimi, Perry G., Chou, Yat-Chen, Franden, Mary Ann, Knoke, Kyle, Tao, Luan, Viitanen, Paul V., Zhang, Min, & Zhang, Yuying. Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate. United States.
Caimi, Perry G., Chou, Yat-Chen, Franden, Mary Ann, Knoke, Kyle, Tao, Luan, Viitanen, Paul V., Zhang, Min, and Zhang, Yuying. Tue . "Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate". United States. https://www.osti.gov/servlets/purl/1014558.
@article{osti_1014558,
title = {Zymomonas with improved ethanol production in medium containing concentrated sugars and acetate},
author = {Caimi, Perry G. and Chou, Yat-Chen and Franden, Mary Ann and Knoke, Kyle and Tao, Luan and Viitanen, Paul V. and Zhang, Min and Zhang, Yuying},
abstractNote = {Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}

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